SDS-Page
BAG2 recombinant protein
BAG2, 1-211aa, Human, His tag, E Coli
Gene Names
BAG2; BAG-2; dJ417I1.2
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
BAG2; 1-211aa; Human; His tag; E Coli; BCL2-associated athanogene 2; BAG-2; BAG2 recombinant protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 0.1M NaCl
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MAQAKINAKA NEGRFCRSSS MADRSSRLLE SLDQLELRVE ALREAATAVE QEKEILLEMI HSIQNSQDMR QISDGEREEL NLTANRLMGR TLTVEVSVET IRNPQQQESL KHATRIIDEV VNKFLDDLGN AKSHLMSLYS ACSSEVPHGP VDQKFQSIVI GCALEDQKKI KRRLETLLRN IENSDKAIKL LEHSKGAGSK TLQQNAESRF N
Sequence Length
211
Applicable Applications for BAG2 recombinant protein
SDS-PAGE
Preparation and Storage
Can be stored at 4°C short term (1-2 weeks).
For long term storage, aliquot and store at -20°C or -70°C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20°C or -70°C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for BAG2 recombinant protein
BCL2-associated athanogene 2, also known as BAG2, is a member of the Bag family of proteins. BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain. BAG2 is a major component of the HSC 70/CHIP chaperone-dependent ubiquitin ligase complex and acts to disrupt CHIP-mediated ubiquitylation. Recombinant human BAG2 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for BAG2 recombinant protein
References
Takayama S., et al. (1999) J Biol Chem. 274:781-786.; Arndt V., et al. (2005) CHIP Mol Biol Cell. 16:5891-5900.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25.9 kDa (231aa) confirmed by MALDI-TOF
NCBI Official Full Name
BAG family molecular chaperone regulator 2
NCBI Official Synonym Full Names
BCL2 associated athanogene 2
NCBI Official Symbol
BAG2
NCBI Official Synonym Symbols
BAG-2; dJ417I1.2
NCBI Protein Information
BAG family molecular chaperone regulator 2
UniProt Protein Name
BAG family molecular chaperone regulator 2
Protein Family
UniProt Gene Name
BAG2
UniProt Synonym Gene Names
BAG-2
NCBI Description
BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq, Jul 2008]
Uniprot Description
Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release (PubMed:24318877, PubMed:9873016).
Research Articles on BAG2
Similar Products
Product Notes
The BAG2 bag2 (Catalog #AAA203762) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's BAG2 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the BAG2 bag2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MAQAKINAKA NEGRFCRSSS MADRSSRLLE SLDQLELRVE ALREAATAVE QEKEILLEMI HSIQNSQDMR QISDGEREEL NLTANRLMGR TLTVEVSVET IRNPQQQESL KHATRIIDEV VNKFLDDLGN AKSHLMSLYS ACSSEVPHGP VDQKFQSIVI GCALEDQKKI KRRLETLLRN IENSDKAIKL LEHSKGAGSK TLQQNAESRF N. It is sometimes possible for the material contained within the vial of "BAG2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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