Human Versican (VS) CLIA Kit | VS clia kit

Human Versican (VS) CLIA Kit

Cat. Num. AAA2000635

• Gene Names: VCAN; WGN; ERVR; GHAP; PG-M; WGN1; CSPG2
• Reactivity: Human
• Synonyms: Versican (VS); Human Versican (VS) CLIA Kit; CSPG2; VCAN; ERVR; PG-M; WGN1; Chondroitin Sulfate Proteoglycan 2; Chondroitin sulfate proteoglycan core protein 2; Glial hyaluronate-binding; Large fibroblast proteoglycan; VS clia kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Versican (VS).; No significant cross-reactivity or interference between Versican (VS) and analogues was observed.
• Sequence Length: 3396

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 27.4-20,000pg/mL
• Sensitivity: < 12.5pg/mL
• Application: Chemiluminescent immunoassay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Versican (VS) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Versican (VS) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for VS clia kit

The microplate provided in this kit has been pre-coated with an antibody specific to Versican (VS). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Versican (VS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Versican (VS) level in the sample or standard.;

Product Categories/Family for VS clia kit

Developmental science; Bone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 21361116
• NCBI GeneID: 1462
• NCBI Accession #: NP_004376.2
• NCBI GenBank Nucleotide #: NM_004385.4
• UniProt Accession #: P13611; P20754; Q13010; Q13189; Q15123; Q9UCL9; Q9UNW5
• Molecular Weight: 369,688 Da
• NCBI Official Full Name: versican core protein isoform 1
• NCBI Official Synonym Full Names: versican
• NCBI Official Symbol: VCAN
• NCBI Official Synonym Symbols: WGN; ERVR; GHAP; PG-M; WGN1; CSPG2
• NCBI Protein Information: versican core protein
• UniProt Protein Name: Versican core protein
• UniProt Gene Name: VCAN
• UniProt Synonym Gene Names: CSPG2; Chondroitin sulfate proteoglycan 2; GHAP
• UniProt Entry Name: CSPG2_HUMAN

NCBI Description

This gene is a member of the aggrecan/versican proteoglycan family. The protein encoded is a large chondroitin sulfate proteoglycan and is a major component of the extracellular matrix. This protein is involved in cell adhesion, proliferation, proliferation, migration and angiogenesis and plays a central role in tissue morphogenesis and maintenance. Mutations in this gene are the cause of Wagner syndrome type 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]

Uniprot Description

CSPG2: May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid. Defects in VCAN are the cause of Wagner syndrome type 1 (WGN1). WGN is a dominantly inherited vitreoretinopathy characterized by an optically empty vitreous cavity with fibrillary condensations and a preretinal avascular membrane. Other optical features include progressive chorioretinal atrophy, perivascular sheating, subcapsular cataract and myopia. Systemic manifestations are absent in WGN. Belongs to the aggrecan/versican proteoglycan family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted

Chromosomal Location of Human Ortholog: 5q14.3

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; lysosomal lumen; intracellular membrane-bound organelle; membrane; Golgi lumen; extracellular region

Molecular Function: protein binding; glycosaminoglycan binding; extracellular matrix structural constituent; hyaluronic acid binding; calcium ion binding; carbohydrate binding

Biological Process: extracellular matrix organization and biogenesis; chondroitin sulfate biosynthetic process; central nervous system development; glycosaminoglycan metabolic process; multicellular organismal development; heart development; cell recognition; pathogenesis; glial cell migration; dermatan sulfate biosynthetic process; osteoblast differentiation; chondroitin sulfate metabolic process; carbohydrate metabolic process; chondroitin sulfate catabolic process; cell adhesion; skeletal development

Disease: Wagner Vitreoretinopathy