Typical Testing Data/Standard Curve (for reference only)
Sheep MHC class I polypeptide-related sequence B ELISA Kit | MICB elisa kit
Sheep MHC class I polypeptide-related sequence B ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: MICB ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MICB antibody and an MICB-HRP conjugate. The assay sample and buffer are incubated together with MICB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MICB concentration since MICB from samples and MICB-HRP conjugate compete for the anti-MICB antibody binding site. Since the number of sites is limited, as more sites are occupied by MICB from the sample, fewer sites are left to bind MICB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MICB concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a heavily glycosylated protein which is a ligand for the NKG2D type II receptor. Binding of the ligand activates the cytolytic response of natural killer (NK) cells, CD8 alphabeta T cells, and gammadelta T cells which express the receptor. This protein is stress-induced and is similar to MHC class I molecules; however, it does not associate with beta-2-microglobulin or bind peptides. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
Uniprot Description
MICB: Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis. Genetic variations in MICA are a cause of susceptibility to rheumatoid arthritis (RA). It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. The MICB*004 allele is associated with rheumatoid arthritis. Genetic variation in MICB is associated with cytomegalovirus and herpes simplex virus I seropositivity and this may be associated with schizophrenia risk. Belongs to the MHC class I family. MIC subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 6p21.3
Cellular Component: cell surface; plasma membrane; integral to membrane
Molecular Function: beta-2-microglobulin binding; antigen binding; natural killer cell lectin-like receptor binding
Biological Process: antigen processing and presentation; regulation of immune response; response to retinoic acid; viral reproduction; response to heat; natural killer cell mediated cytotoxicity; cytolysis; immune response-activating cell surface receptor signaling pathway; negative regulation of defense response to virus by host; response to oxidative stress; gamma-delta T cell activation; T cell mediated cytotoxicity
Research Articles on MICB
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Product Notes
The Sheep MICB micb (Catalog #AAA755232) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA755232 ELISA Kit recognizes Sheep MICB. It is sometimes possible for the material contained within the vial of "MHC class I polypeptide-related sequence B, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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