Human MICB ELISA Kit | MICB elisa kit

Human MICB PicoKine ELISA Kit

Cat. Num. AAA177192

• Gene Names: MICB; PERB11.2
• Reactivity: Human
• Synonyms: MICB; Human MICB PicoKine ELISA Kit; MHC class I polypeptide-related sequence B; MHC class I chain related protein B; MHC class I like molecule PERB11.2 IMX; MHC class I mic B antigen; MHC class I polypeptide related sequence B; MIC B; MIC-B; MICB_HUMAN; PERB11.2; Stress inducible class I homolog; MICB elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human MICB
• Sequence Length: 383

• Samples: Cell culture supernates, cell lysates, serum and plasma (heparin, edta)
• Detection Range: 62.5pg/ml-4000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess interassay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MICB elisa kit

Principle of the Assay: The Picokine™ Human MICB Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human MICB with a 96-well strip plate that is pre-coated with antibody specific for MICB. The detection antibody is a biotinylated antibody specific for MICB. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human MICB with immunogen: Expression system for standard: NSO; Immunogen sequence: A23-G298. The kit is analytically validated with ready to use reagents. To measure Human MICB, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human MICB in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human MICB in the sample.

Background/Introduction: MHC class I polypeptide-related sequence B, also called MICB is a protein that in humans is encoded by the MICB gene. The MICB gene is mapped to 6p21.33. This gene encodes a heavily glycosylated protein which is a ligand for the NKG2D type II receptor. Binding of the ligand activates the cytolytic response of natural killer(NK) cells, CD8 alphabeta T cells, and gammadelta T cells which express the receptor. This protein is stress-induced and is similar to MHC class I molecules; however, it does not associate with beta-2-microglobulin or bind peptides. It seems to have no role in antigen presentation. This gene acts as a stress-induced self-antigen that is recognized by gamma delta T cells. It is the ligand for the KLRK1/NKG2D receptor and binds to KLRK1 leads to cell lysis.

NCBI and Uniprot Product Information

• NCBI GI #: 74706857
• NCBI GeneID: 4277
• UniProt Accession #: Q29980; O14499; O14500; O19798; O19799; O19800; O19801; O19802; A2AC57; A6NP85; B0UZ10; B2RAK2
• Molecular Weight: 4,287 Da
• NCBI Official Full Name: MHC class I polypeptide-related sequence B
• NCBI Official Synonym Full Names: MHC class I polypeptide-related sequence B
• NCBI Official Symbol: MICB
• NCBI Official Synonym Symbols: PERB11.2
• NCBI Protein Information: MHC class I polypeptide-related sequence B; MHC class I mic-B antigen; stress inducible class I homolog; MICB {ECO:0000312|EMBL:CAA62823.1}; MHC class I chain-related protein B; MHC class I-like molecule PERB11.2-IMX
• UniProt Protein Name: MHC class I polypeptide-related sequence B
• Protein Family: MHC class I polypeptide-related sequence
• UniProt Gene Name: MICB
• UniProt Synonym Gene Names: MIC-B
• UniProt Entry Name: MICB_HUMAN

NCBI Description

This gene encodes a heavily glycosylated protein which is a ligand for the NKG2D type II receptor. Binding of the ligand activates the cytolytic response of natural killer (NK) cells, CD8 alphabeta T cells, and gammadelta T cells which express the receptor. This protein is stress-induced and is similar to MHC class I molecules; however, it does not associate with beta-2-microglobulin or bind peptides. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

MICB: Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis. Genetic variations in MICA are a cause of susceptibility to rheumatoid arthritis (RA). It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. The MICB*004 allele is associated with rheumatoid arthritis. Genetic variation in MICB is associated with cytomegalovirus and herpes simplex virus I seropositivity and this may be associated with schizophrenia risk. Belongs to the MHC class I family. MIC subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: cell surface; plasma membrane; integral to membrane

Molecular Function: beta-2-microglobulin binding; antigen binding; natural killer cell lectin-like receptor binding

Biological Process: antigen processing and presentation; regulation of immune response; response to retinoic acid; viral reproduction; response to heat; natural killer cell mediated cytotoxicity; cytolysis; immune response-activating cell surface receptor signaling pathway; response to oxidative stress; negative regulation of defense response to virus by host; gamma-delta T cell activation; T cell mediated cytotoxicity