Mouse MHC class I polypeptide-related sequence B ELISA Kit | MICB elisa kit

Mouse MHC class I polypeptide-related sequence B ELISA Kit

Cat. Num. AAA721736

• Gene Names: MICB; PERB11.2
• Reactivity: Mouse
• Synonyms: MHC class I polypeptide-related sequence B; Mouse MHC class I polypeptide-related sequence B ELISA Kit; MICB elisa kit

• Reactivity: Mouse

• Assay Type: Sandwich
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MICB elisa kit

Intended Uses: This MHC I/H-2 III ELISA kit is intended Laboratory for research use only.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofMHC I/H-2 III in the sample, thisMHC I/H-2 III ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus MHC I/H-2 III concentration. The concentration of MHC I/H-2 III in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: This MHC I/H-2 III enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for MHC I/H-2 III. Standards or samples are then added to the microtiter plate wells and MHC I/H-2 III if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of MHC I/H-2 III present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for MHC I/H-2 III are added to each well to "sandwich" the MHC I/H-2 III immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that contain MHC I/H-2 III and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.

Product Categories/Family for MICB elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 167857788
• NCBI GeneID: 4277
• NCBI Accession #: NP_005922.2
• NCBI GenBank Nucleotide #: NM_005931.4
• Molecular Weight: 4,287 Da
• NCBI Official Full Name: MHC class I polypeptide-related sequence B isoform 1
• NCBI Official Synonym Full Names: MHC class I polypeptide-related sequence B
• NCBI Official Symbol: MICB
• NCBI Official Synonym Symbols: PERB11.2
• NCBI Protein Information: MHC class I polypeptide-related sequence B; MHC class I mic-B antigen; stress inducible class I homolog; MHC class I chain-related protein B; MHC class I-like molecule PERB11.2-IMX
• UniProt Protein Name: MHC class I polypeptide-related sequence B
• Protein Family: MHC class I polypeptide-related sequence
• UniProt Gene Name: MICB
• UniProt Synonym Gene Names: PERB11.2; MIC-B
• UniProt Entry Name: MICB_HUMAN

NCBI Description

This gene encodes a heavily glycosylated protein which is a ligand for the NKG2D type II receptor. Binding of the ligand activates the cytolytic response of natural killer (NK) cells, CD8 alphabeta T cells, and gammadelta T cells which express the receptor. This protein is stress-induced and is similar to MHC class I molecules; however, it does not associate with beta-2-microglobulin or bind peptides. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

MICB: Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis. Genetic variations in MICA are a cause of susceptibility to rheumatoid arthritis (RA). It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. The MICB*004 allele is associated with rheumatoid arthritis. Genetic variation in MICB is associated with cytomegalovirus and herpes simplex virus I seropositivity and this may be associated with schizophrenia risk. Belongs to the MHC class I family. MIC subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: cell surface; plasma membrane; integral to membrane

Molecular Function: beta-2-microglobulin binding; antigen binding; natural killer cell lectin-like receptor binding

Biological Process: antigen processing and presentation; regulation of immune response; response to retinoic acid; viral reproduction; natural killer cell mediated cytotoxicity; response to heat; cytolysis; immune response-activating cell surface receptor signaling pathway; response to oxidative stress; negative regulation of defense response to virus by host; gamma-delta T cell activation; T cell mediated cytotoxicity