Human MHC class I polypeptide-related sequence B ELISA Kit | MICB elisa kit

Human MHC class I polypeptide-related sequence B, MICB ELISA Kit

Cat. Num. AAA945571

• Gene Names: MICB; PERB11.2
• Reactivity: Human
• Synonyms: MHC class I polypeptide-related sequence B; Human MHC class I polypeptide-related sequence B; MICB ELISA Kit; Human MHC class I polypeptide-related sequence B (MICB) ELISA kit; DAAP-210H10.1; PERB11.2; MHC class I chain-related protein B; MHC class I mic-B antigen; MHC class I molecule; MHC class I-like molecule PERB11.2-IMX; stress inducible class I homolog; MICB elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human MICB. No significant cross-reactivity or interference between Human MICB and analogues was observed.
• Sequence Length: 351

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 31.25 pg/ml-2000 pg/ml
• Sensitivity: 7.8 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Immunity
• Protein Biological Process 3: Adaptive immunity
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MICB elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MICB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MICB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MICB is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MICB bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 574584674
• NCBI GeneID: 4277
• NCBI Accession #: NP_001276089.1
• NCBI GenBank Nucleotide #: NM_001289160.1
• UniProt Accession #: Q29980; O14499; O14500; O19798; O19799; O19800; O19801; O19802; A2AC57; A6NP85; B0UZ10; B2RAK2
• Molecular Weight: 42,646 Da
• NCBI Official Full Name: MHC class I polypeptide-related sequence B isoform 2
• NCBI Official Synonym Full Names: MHC class I polypeptide-related sequence B
• NCBI Official Symbol: MICB
• NCBI Official Synonym Symbols: PERB11.2
• NCBI Protein Information: MHC class I polypeptide-related sequence B; MHC class I mic-B antigen; stress inducible class I homolog; MHC class I chain-related protein B; MHC class I-like molecule PERB11.2-IMX
• UniProt Protein Name: MHC class I polypeptide-related sequence B
• Protein Family: MHC class I polypeptide-related sequence
• UniProt Gene Name: MICB
• UniProt Synonym Gene Names: PERB11.2; MIC-B
• UniProt Entry Name: MICB_HUMAN

NCBI Description

This gene encodes a heavily glycosylated protein which is a ligand for the NKG2D type II receptor. Binding of the ligand activates the cytolytic response of natural killer (NK) cells, CD8 alphabeta T cells, and gammadelta T cells which express the receptor. This protein is stress-induced and is similar to MHC class I molecules; however, it does not associate with beta-2-microglobulin or bind peptides. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

MICB: Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis. Genetic variations in MICA are a cause of susceptibility to rheumatoid arthritis (RA). It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. The MICB*004 allele is associated with rheumatoid arthritis. Genetic variation in MICB is associated with cytomegalovirus and herpes simplex virus I seropositivity and this may be associated with schizophrenia risk. Belongs to the MHC class I family. MIC subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: cell surface; integral to membrane; plasma membrane

Molecular Function: beta-2-microglobulin binding; antigen binding; natural killer cell lectin-like receptor binding

Biological Process: antigen processing and presentation; regulation of immune response; response to retinoic acid; viral reproduction; natural killer cell mediated cytotoxicity; response to heat; cytolysis; immune response-activating cell surface receptor signaling pathway; response to oxidative stress; negative regulation of defense response to virus by host; gamma-delta T cell activation; T cell mediated cytotoxicity