Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Myogenic factor 5 (MYF5) Standard Curve.)
Human Myogenic Factor 5 (MYF5) ELISA Kit | MYF5 elisa kit
Human Myogenic Factor 5 (MYF5) ELISA Kit
Gene Names
MYF5; EORVA; bHLHc2
Reactivity
Human
Synonyms
Myogenic Factor 5 (MYF5); Human Myogenic Factor 5 (MYF5) ELISA Kit; MYF5; bHLHc2; MYF5 elisa kit
Reactivity
Human
Specificity
Human Myogenic factor 5 (MYF5) ELISA Kit has high sensitivity and excellent specificity for detection of Human MYF5. No significant cross-reactivity or interference between Human MYF5 and analogues was observed.
Sequence Length
255
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Myogenic factor 5 (MYF5) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Myogenic factor 5 (MYF5) Standard Curve.)
Related Product Information for MYF5 elisa kit
Description: This Human Myogenic factor 5 (MYF5) ELISA Kit employs a two-site sandwich ELISA to quantitate MYF5 in samples. An antibody specific for MYF5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MYF5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYF5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYF5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: MYF5 (Myogenic Factor 5) is a Protein Coding gene. Diseases associated with MYF5 include Rhabdomyosarcoma and Skeletal Muscle Cancer. Among its related pathways are Transcriptional Regulatory Network in Embryonic Stem Cell and CDO in myogenesis. GO annotations related to this gene include sequence-specific DNA binding and RNA polymerase II core promoter proximal region sequence-specific DNA binding. An important paralog of this gene is MYOD1. Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYOG and MYOD1, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.
Background: MYF5 (Myogenic Factor 5) is a Protein Coding gene. Diseases associated with MYF5 include Rhabdomyosarcoma and Skeletal Muscle Cancer. Among its related pathways are Transcriptional Regulatory Network in Embryonic Stem Cell and CDO in myogenesis. GO annotations related to this gene include sequence-specific DNA binding and RNA polymerase II core promoter proximal region sequence-specific DNA binding. An important paralog of this gene is MYOD1. Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYOG and MYOD1, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
myogenic factor 5
NCBI Official Synonym Full Names
myogenic factor 5
NCBI Official Symbol
MYF5
NCBI Official Synonym Symbols
EORVA; bHLHc2
NCBI Protein Information
myogenic factor 5
UniProt Protein Name
Myogenic factor 5
Protein Family
UniProt Gene Name
MYF5
UniProt Synonym Gene Names
BHLHC2; Myf-5; bHLHc2
UniProt Entry Name
MYF5_HUMAN
Uniprot Description
Myf-5: Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.
Protein type: Transcription factor; DNA-binding
Chromosomal Location of Human Ortholog: 12q21
Cellular Component: nucleoplasm
Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; protein heterodimerization activity
Biological Process: transcription from RNA polymerase II promoter; ossification; extracellular matrix organization and biogenesis; muscle development; skeletal muscle development; somitogenesis; camera-type eye development; regulation of cell-matrix adhesion; muscle cell fate commitment; positive regulation of myoblast differentiation; embryonic skeletal morphogenesis; regulation of transcription from RNA polymerase II promoter; muscle cell differentiation; positive regulation of skeletal muscle fiber development; positive regulation of muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; cartilage condensation
Research Articles on MYF5
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Product Notes
The Human MYF5 myf5 (Catalog #AAA9715112) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715112 ELISA Kit recognizes Human MYF5. It is sometimes possible for the material contained within the vial of "Myogenic Factor 5 (MYF5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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