MFI2 Recombinant Protein | MELTF recombinant protein

MFI2 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8123081

• Gene Names: Mfi2; MTf; CD228
• Purity: >95% as determined by SDS-PAGE
• Synonyms: MFI2; MFI2 Protein; Mouse; Recombinant (His Tag); Mouse MFI2/CD228/melanotransferrin Protein (His Tag); CD228 Protein; MTf Protein; melanotransferrin; MELTF recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gln708

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Val 20
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse MFI2 (Q9R0R1)(Met 1-Gln 708), without the propeptide, was fused with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for MELTF recombinant protein

Background: MELTF (Melanotransferrin, also known as MFI2) is a Protein Coding gene. The protein encoded by this gene is a cell-surface glycoprotein found on melanoma cells. The protein shares sequence similarity and iron-binding properties with members of the transferrin superfamily. MELTF is broadly expressed in the salivary gland, kidney, and other tissues. The long non-coding RNA MFI2 antisense RNA is overexpressed in human cancer tissues and its increased expression is associated with the occurrence and metastasis of cancer. lncRNA MFI2 could promote proliferation and migration of osteosarcoma cells by regulating FOXP4 expression, which suggested critical roles of lncRNA MFI2 and FOXP4 in the occurrence and development of human osteosarcoma. Diseases associated with MELTF include Osteogenic Sarcoma and Melanotic Neuroectodermal Tumor.

References

Richardson DR. (2000) The role of the membrane-bound tumour antigen, melanotransferrin (p97), in iron uptake by the human malignant melanoma cell. Eur J Biochem. 267(5): 1290-8.Kim DK, et al. (2001) Serum melanotransferrin, p97 as a biochemical marker of Alzheimer's disease. Neuropsychopharmacology. 25(1): 84-90.Rothenberger S, et al. (1996) Coincident expression and distribution of melanotransferrin and transferrin receptor in human brain capillary endothelium. Brain Res. 712(1): 117-21.Baker EN, et al. (1992) Human melanotransferrin (p97) has only one functional iron-binding site. FEBS Lett. 298(2-3): 215-8.

NCBI and Uniprot Product Information

• NCBI GI #: 13994140
• NCBI GeneID: 30060
• NCBI Accession #: NP_038928.1
• NCBI GenBank Nucleotide #: NM_013900.2
• UniProt Accession #: Q9R0R1
• Molecular Weight: 81,293 Da
• NCBI Official Full Name: melanotransferrin
• NCBI Official Synonym Full Names: antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5
• NCBI Official Symbol: Mfi2
• NCBI Official Synonym Symbols: MTf; CD228
• NCBI Protein Information: melanotransferrin; membrane-bound transferrin-like protein p97
• UniProt Protein Name: Melanotransferrin
• Protein Family: Melanotransferrin
• UniProt Gene Name: Mfi2
• UniProt Synonym Gene Names: Mtf; MTf
• UniProt Entry Name: TRFM_MOUSE