MED20 recombinant protein

Purified recombinant Human MED20 protein (His tag)

Cat. Num. AAA5308819

• Gene Names: MED20; TRFP; PRO0213
• Applications: SDS-Page
• Purity: > 90% pure
• Synonyms: MED20; Purified recombinant Human MED20 protein (His tag); MED20 protein (His tag); MED20 recombinant protein

• Host: Human
• Purity/Purification: > 90% pure
• Form/Format: Supplied in liquid form in 20mM Tris-HCl buffer (pH 8.5) containing 0.2M NaCl, 50% glycerol, 1mM DTT
• Concentration: 250 ug/ml (varies by lot)
• Sequence: 1-149aa: MGSSHHHHHH SSGLVPRGSH MGSMGVTCVS QMPVAEGKSV QQTVELLTRK LEMLGAEKQG TFCVDCETYH TAASTLGSQG QTGKLMYVMH NSEYPLSCFA LFENGPCLIA DTNFDVLMVK LKGFFQSAKA SKIETRGTRY QYCDFLVKVG TVTMGPSARG ISVEVEYGPC VVASDCWSLL LEFLQSFLGS HTPGAPAVFG NRHDAVYGPA DTMVQYMELF NKIRKQQQVP VAGIR
• Sequence Length: 123

• Applicable Applications for MED20 recombinant protein: SDS-PAGE
• Expression: E Coli
• Tag: His tag
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

SDS-Page

(Figure annotation denotes ug of protein loaded and % gel used.)

Product Categories/Family for MED20 recombinant protein

DNA & RNA

NCBI and Uniprot Product Information

• NCBI GI #: 768711651
• NCBI GeneID: 9477
• NCBI Accession #: NP_001292386.1
• NCBI GenBank Nucleotide #: NM_001305457.1
• UniProt Accession #: Q9H944; O95821; Q5T8J4; Q9Y429; B4DE08
• Molecular Weight: 13,540 Da
• NCBI Official Full Name: mediator of RNA polymerase II transcription subunit 20 isoform 3
• NCBI Official Synonym Full Names: mediator complex subunit 20
• NCBI Official Symbol: MED20
• NCBI Official Synonym Symbols: TRFP; PRO0213
• NCBI Protein Information: mediator of RNA polymerase II transcription subunit 20
• UniProt Protein Name: Mediator of RNA polymerase II transcription subunit 20
• Protein Family: Putative mediator of RNA polymerase II transcription
• UniProt Gene Name: MED20
• UniProt Synonym Gene Names: TRFP; hTRFP

NCBI Description

This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. A mutation in this gene has been associated with a novel infantile-onset neurodegenerative movement disorder. Alternatively spliced transcript variants have been identified. [provided by RefSeq, Mar 2015]

Uniprot Description

TRFP: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 20 family.

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: nucleoplasm; Srb-mediator complex

Molecular Function: protein binding; transcription coactivator activity

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter