Western Blot (WB)
(Western blot analysis of Dynein IC1 using COLO205 whole cell lysates)
Rabbit anti-Human Dynein IC1 Polyclonal Antibody | anti-DNAI1 antibody
Dynein IC1 Antibody
Gene Names
DNAI1; PCD; DIC1; ICS1; CILD1
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
Dynein IC1; Polyclonal Antibody; Dynein IC1 Antibody; Axonemal dynein intermediate chain 1; Axonemal dynein intermediate chain 2; CILD 1; CILD1; Cytoplasmic dynein 1 intermediate chain 1; Cytoplasmic dynein 1 intermediate chain 2; Cytoplasmic dynein intermediate chain 1; Cytoplasmic dynein intermediate chain 2; DH IC 1; DH IC 2; DIC1; DNAI 1; DNAI 2; DNAI1; DNAI1_HUMAN; DNAI2; DNCI 2; DNCI1; DNCI2; DNCIC 1; DNCIC 2; DNCIC1; DNCIC2; DYNC1I1; DYNC1I2; Dynein axonemal intermediate chain 1; Dynein axonemal intermediate polypeptide 1; Dynein axonemal intermediate polypeptide 2; Dynein cytoplasmic intermediate polypeptide 1; Dynein cytoplasmic intermediate polypeptide 2; Dynein intermediate chain 1 axonemal; Dynein intermediate chain 1 cytosolic; Dynein intermediate chain 1; axonemal; Dynein intermediate chain 2 axonemal; Dynein intermediate chain 2 cytosolic; Dynein intermediate chain DNAI1; IC74; ICS; ICS1; Immotile cilia syndrome 1; MGC26204; PCD; anti-DNAI1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Dynein IC1 antibody detects endogenous levels of total Dynein IC1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
703
Applicable Applications for anti-DNAI1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide derived from human Dynein IC1
Subcellular Location
Cytoskeleton
Predicted Cross Reactivity
Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Similarity
Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (92%), Xenopus (83%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of Dynein IC1 using COLO205 whole cell lysates)
Western Blot (WB)
(Western blot analysis of Dynein IC1 using COLO205 whole cell lysates)
Immunofluorescene (IF)
(MBS9602099 staining COLO205 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescene (IF)
(MBS9602099 staining COLO205 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-DNAI1 antibody
Function: Part of the dynein complex of respiratory cilia.
Subunit Structure: Consists of at least two heavy chains and a number of intermediate and light chains. Interacts with BICD2.
Similarity: Belongs to the dynein intermediate chain family.
Subunit Structure: Consists of at least two heavy chains and a number of intermediate and light chains. Interacts with BICD2.
Similarity: Belongs to the dynein intermediate chain family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 79 kDa
Predicted: 80 kDa
Predicted: 80 kDa
NCBI Official Full Name
dynein intermediate chain 1, axonemal isoform 2
NCBI Official Synonym Full Names
dynein axonemal intermediate chain 1
NCBI Official Symbol
DNAI1
NCBI Official Synonym Symbols
PCD; DIC1; ICS1; CILD1
NCBI Protein Information
dynein intermediate chain 1, axonemal
UniProt Protein Name
Dynein intermediate chain 1, axonemal
Protein Family
UniProt Gene Name
DNAI1
NCBI Description
This gene encodes a member of the dynein intermediate chain family. The encoded protein is part of the dynein complex in respiratory cilia. The inner- and outer-arm dyneins, which bridge between the doublet microtubules in axonemes, are the force-generating proteins responsible for the sliding movement in axonemes. The intermediate and light chains, thought to form the base of the dynein arm, help mediate attachment and may also participate in regulating dynein activity. Mutations in this gene result in abnormal ciliary ultrastructure and function associated with primary ciliary dyskinesia and Kartagener syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
Uniprot Description
Part of the dynein complex of respiratory cilia.
Research Articles on DNAI1
Similar Products
Product Notes
The DNAI1 dnai1 (Catalog #AAA9602099) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Dynein IC1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Dynein IC1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the DNAI1 dnai1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Dynein IC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
