MED20 recombinant protein

MED20, 1-212aa, Human, His tag, E Coli

Cat. Num. AAA204934

• Gene Names: MED20; SRB2; TRFP; PRO0213
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: MED20; 1-212aa; Human; His tag; E Coli; Mediator of RNA polymerase II transcription subunit 20; PRO0213; TRFP; MED20 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.5) containing 0.2M NaCl, 50% glycerol, 1mM DTT
• Concentration: 0.25 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMGVTCVS QMPVAEGKSV QQTVELLTRK LEMLGAEKQG TFCVDCETYH TAASTLGSQG QTGKLMYVMH NSEYPLSCFA LFENGPCLIA DTNFDVLMVK LKGFFQSAKA SKIETRGTRY QYCDFLVKVG TVTMGPSARG ISVEVEYGPC VVASDCWSLL LEFLQSFLGS HTPGAPAVFG NRHDAVYGPA DTMVQYMELF NKIRKQQQVP VAGIR
• Sequence Length: 212

• Applicable Applications for MED20 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for MED20 recombinant protein

MED20 is a subunit of the Mediator complex, a multiprotein coactivator of RNA transcription that interacts with DNA-bound transcriptional activators, RNA polymerase II (see MIM 180660), and general initiation factors. Recombinant human MED20 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for MED20 recombinant protein

Epigenomics

References

Sato S, Tomomori-Sato C, et al. (2004). Mol Cell. 14 (5):685-91.; Zhang X, Krutchinsky A, et al. (2011). Mol Cell. 19 (1):89-100.;

NCBI and Uniprot Product Information

• NCBI GI #: 30023851
• NCBI GeneID: 9477
• NCBI Accession #: NP_004266
• NCBI GenBank Nucleotide #: NM_004275.4
• UniProt Accession #: Q9H944; O95821; Q5T8J4; Q9Y429; B4DE08
• Molecular Weight: 25 kDa (235aa), confirmed by MALDI-TOF
• NCBI Official Full Name: mediator of RNA polymerase II transcription subunit 20 isoform 1
• NCBI Official Synonym Full Names: mediator complex subunit 20
• NCBI Official Symbol: MED20
• NCBI Official Synonym Symbols: SRB2; TRFP; PRO0213
• NCBI Protein Information: mediator of RNA polymerase II transcription subunit 20
• UniProt Protein Name: Mediator of RNA polymerase II transcription subunit 20
• Protein Family: Putative mediator of RNA polymerase II transcription
• UniProt Gene Name: MED20
• UniProt Synonym Gene Names: TRFP; hTRFP

NCBI Description

This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. A mutation in this gene has been associated with a novel infantile-onset neurodegenerative movement disorder. Alternatively spliced transcript variants have been identified. [provided by RefSeq, Mar 2015]

Uniprot Description

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.