CD146 / MCAM recombinant protein

Recombinant Human CD146 / MCAM Protein (His tag)

Cat. Num. AAA2546512

• Gene Names: MCAM; CD146; MUC18
• Purity: > 98 % as determined by reducing SDS-PAGE.
• Synonyms: CD146 / MCAM; Recombinant Human CD146 / MCAM Protein (His tag); CD146; MUC18; CD146 / MCAM recombinant protein

• Host: HEK293 Cells
• Purity/Purification: > 98 % as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from sterile PBS, pH 7.4, 5% trehalose, 5% mannitol, 0.01% tween-80.
• Sequence: Met 1-Gly 559

• Species: Human
• Tag: C-His
• Endotoxin: < 1.0 EU per ug as determined by the LAL method.
• Reconstitution: We recommend that the vial be centrifuged to opening to bring the contents to the bottom. Do not mix by vortex. It is recommended that sterile water(70 ul) be added to the vial to prepare a stock solution of 0.15 mg/mL. Concentration is measured by UV-Vis.
• Preparation and Storage: Storage: Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.; ; Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

SDS-PAGE

Related Product Information for CD146 / MCAM recombinant protein

The CD146 antigen, also known as melanoma cell adhesion molecule (MCAM) and MUC18, is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. CD146 contains the characteristic immunoglobulin-like domains (V-V-C2-C2-C2), a transmembrane region and a short cytoplasmic tail. The CD146 expression is detected in endothelial cells in vascular tissue throughout the body, and plays a role in cell adhesion, as well as in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. As a Ca2+-independent cell adhesion molecule involved in heterophilic cell to cell interactions and a surface receptor, CD146 triggers tyrosine phosphorylation of FYN and PTK2 and subsequently induced signal transduction, proteolysis, or immune recognition. This protein is also expressed predominantly on metastatic lesions and advanced primary tumours, and thus has been suggested to play an important role in tumour progression and the development of metastasis in certain human carcinomas.

NCBI and Uniprot Product Information

• NCBI GI #: 71274107
• NCBI GeneID: 4162
• NCBI Accession #: NP_006491.2
• NCBI GenBank Nucleotide #: NM_006500.2
• Molecular Weight: Molecular Weight: 61.3 kDa; AP Molecular Weight: 80-90 kDa
• NCBI Official Full Name: cell surface glycoprotein MUC18
• NCBI Official Synonym Full Names: melanoma cell adhesion molecule
• NCBI Official Symbol: MCAM
• NCBI Official Synonym Symbols: CD146; MUC18
• NCBI Protein Information: cell surface glycoprotein MUC18
• UniProt Protein Name: Cell surface glycoprotein MUC18
• UniProt Gene Name: MCAM
• UniProt Synonym Gene Names: MUC18

Uniprot Description

MCAM: Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: external side of plasma membrane; focal adhesion

Biological Process: anatomical structure morphogenesis; angiogenesis; glomerular filtration