Rabbit anti-Human RLN2 Polyclonal Antibody | anti-RLN2 antibody

RLN2, CT (RLN2, Prorelaxin H2, Relaxin B chain, Relaxin A chain) (AP)

Cat. Num. AAA6342258

• Gene Names: RLN2; H2; RLXH2; H2-RLX; bA12D24.1.1; bA12D24.1.2
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RLN2; Polyclonal Antibody; CT (RLN2; Prorelaxin H2; Relaxin B chain; Relaxin A chain) (AP); Relaxin A chain; anti-RLN2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-RLN2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RLN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 138-165 amino acids from the C-terminal region of human RLN2.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(RLN2 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the RLN2 antibody detected the RLN2 protein (arrow).)

Related Product Information for anti-RLN2 antibody

Relaxins are known endocrine and autocrine/paracrine hormones, belonging to the insulin gene superfamily. In the human there are three non-allelic relaxin genes, RLN1, RLN2 and RLN3. RLN1 and RLN2 share high sequence homology. The active form of the encoded protein consists of an A chain and a B chain but their cleavage sites are not definitely described yet. Relaxin is produced by the ovary, and targets the mammalian reproductive system to ripen the cervix, elongate the pubic symphysis and inhibit uterine contraction. It may have additional roles in enhancing sperm motility, regulating blood pressure, controlling heart rate and releasing oxytocin and vasopressin. There are two alternatively spliced transcript variants encoding different isoforms described for this gene.

Product Categories/Family for anti-RLN2 antibody

Antibodies; Spermatogenesis; Testis Proteins

References

Davila, S., et al. Genes Immun. 11(3):232-238(2010). Vogel, I., et al. In Vivo 23(6):1005-1009(2009). Dschietzig, T., et al. Regul. Pept. 155 (1-3), 163-173 (2009): Mookerjee, I., et al. FASEB J. 23(4):1219-1229(2009). Svendsen, A.M., et al. Mol. Cell. Endocrinol. 296 (1-2), 10-17 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 20143935
• NCBI GeneID: 6019
• NCBI Accession #: NP_005050.2
• NCBI GenBank Nucleotide #: NM_005059.3
• UniProt Accession #: P04090; Q99936; Q9UCX3; Q9UQJ2; A0AVM0
• Molecular Weight: 12,883 Da
• NCBI Official Full Name: prorelaxin H2 isoform 2
• NCBI Official Synonym Full Names: relaxin 2
• NCBI Official Symbol: RLN2
• NCBI Official Synonym Symbols: H2; RLXH2; H2-RLX; bA12D24.1.1; bA12D24.1.2
• NCBI Protein Information: prorelaxin H2; H2-preprorelaxin; relaxin, ovarian, of pregnancy
• UniProt Protein Name: Prorelaxin H2
• Protein Family: Prorelaxin
• UniProt Gene Name: RLN2
• UniProt Entry Name: REL2_HUMAN

NCBI Description

Relaxins are known endocrine and autocrine/paracrine hormones, belonging to the insulin gene superfamily. In humans there are three non-allelic relaxin genes, RLN1, RLN2 and RLN3, where RLN1 and RLN2 share high sequence homology. The active form of the protein encoded by this gene consists of an A chain and a B chain linked by disulfide bonds. Relaxin is produced by the ovary, and targets the mammalian reproductive system to ripen the cervix, elongate the pubic symphysis and inhibit uterine contraction. It may have additional roles in enhancing sperm motility, regulating blood pressure, controlling heart rate and releasing oxytocin and vasopressin. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2013]

Uniprot Description

RLN2: Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals. May be involved in remodeling of connective tissues during pregnancy, promoting growth of pubic ligaments and ripening of the cervix. Belongs to the insulin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 9p24.1

Cellular Component: extracellular region

Molecular Function: hormone activity

Biological Process: female pregnancy