Microtubule Associated Serine/Threonine Kinase 2 Recombinant Protein | MAST2 recombinant protein

Recombinant Microtubule Associated Serine/Threonine Kinase 2 (MAST2)

Cat. Num. AAA2029086

• Gene Names: MAST2; MTSSK; MAST205
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >90%
• Synonyms: Microtubule Associated Serine/Threonine Kinase 2; Recombinant Microtubule Associated Serine/Threonine Kinase 2 (MAST2); MAST2 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >90%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; FETIKLISN GAYGAVFLVR HKSTRQRFAM KKINKQNLILRNQIQQAFVE RDILTFAENP FVVSMFCSFD TKRHLCMVME YVEGGDCATLLKNIGALPVD MVRLYFAETV LALEYLHNYG IVHRDLKPDN LLITSMGHIKLTDFGLSKIG LMSLTTNLYE GHIEKDAREF LDKQVCGTPE YIAPEVILRQGYGKPVDWWA MGIILYEFLV GCVPFFGDTP EELFGQVISD EIVWPEGDEALPPDAQDLTS KLLHQNPLER LGTGSAYEVK QHPFF
• Sequence Length: 1797

• Applicable Applications for MAST2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 61.2kDa
• Accurate Molecular Mass (KD): 61kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and S-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Phe512~Phe785
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 41350925
• NCBI GeneID: 23139
• Molecular Weight: 176,132 Da
• NCBI Official Full Name: Microtubule associated serine/threonine kinase 2
• NCBI Official Synonym Full Names: microtubule associated serine/threonine kinase 2
• NCBI Official Symbol: MAST2
• NCBI Official Synonym Symbols: MTSSK; MAST205
• NCBI Protein Information: microtubule-associated serine/threonine-protein kinase 2
• UniProt Protein Name: Microtubule-associated serine/threonine-protein kinase 2
• Protein Family: Microtubule-associated serine/threonine-protein kinase
• UniProt Gene Name: MAST2
• UniProt Entry Name: MAST2_HUMAN

Uniprot Description

MAST2: Appears to link the dystrophin/utrophin network with microtubule filaments via the syntrophins. Phosphorylation of DMD or UTRN may modulate their affinities for associated proteins. Functions in a multi-protein complex in spermatid maturation. Regulates lipopolysaccharide-induced IL-12 synthesis in macrophages by forming a complex with TRAF6, resulting in the inhibition of TRAF6 NF-kappa-B activation. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, AGC; Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); AGC group; MAST family

Chromosomal Location of Human Ortholog: 1p34.1

Cellular Component: microtubule cytoskeleton; cytoplasm; plasma membrane

Molecular Function: protein serine/threonine kinase activity; protein binding; microtubule binding; magnesium ion binding; ATP binding; phosphatase binding

Biological Process: spermatid differentiation; regulation of interleukin-12 biosynthetic process; protein amino acid phosphorylation