Microtubule-associated protein tau (Mapt) Recombinant Protein | Mapt recombinant protein

Recombinant Rat Microtubule-associated protein tau (Mapt)

Cat. Num. AAA965317

• Gene Names: Mapt; Tau; pTau; Mtapt; RNPTAU
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Microtubule-associated protein tau (Mapt); Recombinant Rat Microtubule-associated protein tau (Mapt); Neurofibrillary tangle protein; Paired helical filament-tau; PHF-tau; Mapt recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 2-752aa; Full Length of Mature Protein
• Sequence: AEPRQEFDTMEDQAGDYTMLQDQEGDMDHGLKESPPQPPADDGSEEPGSETSDAKSTPTAEDVTAPLVEERAPDKQATAQSHTEIPEGTTAEEAGIGDTPNMEDQAAGHVTQEPQKVEIFSQSLLVEPGRREGQAPDSGISDWTHQQVPSMSGAPLPPQGLREATHQPLGTRPEDVERSHPASELLWQESPQKEAWGKDRLGSEEEVDEDITMDESSQESPPSQASLAPGTATPQARSVSASGVSGETTSIPGFPAEGSIPLPADFFSKVSAETQASPPEGPGTGPSEEGHEAAPEFTFHVEIKASAPKEQDLEGATVVGAPAEEQKARGPSVGKGTKEASLLEPTDKQPAAGLPGRPVSRVPQLKARVAGVSKDRTGNDEKKAKTSTPSCAKTPSNRPCLSPTRPTPGSSDPLIKPSSPAVCPEPATSPKYVSSVTPRNGSPGTKQMKLKGADGKTGAKIATPRGAATPGQKGTSNATRIPAKTTPSPKTPPGSGEPPKSGERSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSASKSRLQTAPVPMPDLKNVRSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVPGGGSVHIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL

• Production Note: Special Offer: The Yeast host-expressed protein is manufactured from a stock plasmid containing the protein gene. Yeasthost-expressed protein is stocked in different unit sizes ranging from as small as 10 ug to as large as 1 mg. Bulk inventory is also available. The Yeast host-expressed protein has been ordered over and over again by researchers and has stood the test of time as both a robust protein and important target for the research community. It is part of our new program to make our most popular protein targets and corresponding hosts available in expanded unit sizes and with a quick processing time. Select Yeast host-expressed protein for the fastest delivery among all hosts. Please contact our technical support team or email to [email protected] for more details.
• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

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Related Product Information for Mapt recombinant protein

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

References

Cloning of a big tau microtubule-associated protein characteristic of the peripheral nervous system.Goedert M., Spillantini M.G., Crowther R.A.Proc. Natl. Acad. Sci. U.S.A. 89:1983-1987(1992) Expression of high molecular weight tau in the central and peripheral nervous systems.Georgieff I.S., Liem R.K.H., Couchie D., Mavilia C., Nunez J., Shelanski M.L.J. Cell Sci. 105:729-737(1993) Complete sequence of 3'-untranslated region of tau from rat central nervous system. Implications for mRNA heterogeneity.Sadot E., Marx R., Barg J., Behar L., Ginzburg I.J. Mol. Biol. 241:325-331(1994) Developmentally regulated expression of specific tau sequences.Kosik K.S., Orecchio L.D., Bakalis S., Neve R.L.Neuron 2:1389-1397(1989) Expression of multiple tau isoforms and microtubule bundle formation in fibroblasts transfected with a single tau cDNA.Kanai Y., Takemura R., Oshima T., Mori H., Ihara Y., Yanagisawa M., Masaki T., Hirokawa N.J. Cell Biol. 109:1173-1184(1989) Diversity of high-molecular-weight tau proteins in different regions of the nervous system.Mavilia C., Couchie D., Nunez J.J. Neurochem. 63:2300-2306(1994) In vivo phosphorylation sites in fetal and adult rat tau.Watanabe A., Hasegawa M., Suzuki M., Takio K., Morishima-Kawashima M., Titani K., Arai T., Kosik K.S., Ihara Y.J. Biol. Chem. 268:25712-25717(1993) Molecular diversity at the carboxyl terminus of human and rat tau.Sawa A., Oyama F., Matsushita M., Ihara Y.Brain Res. Mol. Brain Res. 27:111-117(1994) A role for FKBP52 in Tau protein function.Chambraud B., Sardin E., Giustiniani J., Dounane O., Schumacher M., Goedert M., Baulieu E.E.Proc. Natl. Acad. Sci. U.S.A. 107:2658-2663(2010)

NCBI and Uniprot Product Information

• NCBI GI #: 13432197
• NCBI GeneID: 29477
• UniProt Accession #: P19332; Q63567; Q63677; Q9QW06
• Molecular Weight: 80.4 kDa
• NCBI Official Full Name: Microtubule-associated protein tau
• NCBI Official Synonym Full Names: microtubule-associated protein tau
• NCBI Official Symbol: Mapt
• NCBI Official Synonym Symbols: Tau; pTau; Mtapt; RNPTAU
• NCBI Protein Information: microtubule-associated protein tau
• UniProt Protein Name: Microtubule-associated protein tau
• Protein Family: Microtubule-associated protein
• UniProt Gene Name: Mapt
• UniProt Synonym Gene Names: Mtapt; Tau; PHF-tau
• UniProt Entry Name: TAU_RAT

NCBI Description

a microtubule-associated protein; expression is found specifically in neurons [RGD, Feb 2006]

Uniprot Description

Tau: a microtubule-associated protein that regulates microtubule assembly and stability. Apparently involved in the establishment and maintenance of neuronal polarity. Mutations can result in several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. Nine differentially spliced isoforms have been described. The short isoforms allow plasticity of the cytoskeleton, whereas the longer isoforms may preferentially play a role in its stabilization.

Protein type: Cytoskeletal

Cellular Component: axon; axoneme; cytoplasm; cytosol; dendrite; growth cone; microtubule; microtubule associated complex; microtubule cytoskeleton; neuron projection; nucleus; plasma membrane; postsynaptic density; tubulin complex

Molecular Function: apolipoprotein binding; enzyme binding; Hsp90 protein binding; microtubule binding; protein binding; protein complex binding; protein domain specific binding; protein kinase binding; protein phosphatase 2A binding; SH3 domain binding

Biological Process: adult walking behavior; apoptosis; axon cargo transport; axon extension; axonogenesis; brain development; female pregnancy; induction of apoptosis by oxidative stress; memory; microtubule cytoskeleton organization and biogenesis; microtubule-based process; mitochondrion transport along microtubule; negative regulation of intracellular transport; neurite development; neuron differentiation; neuron migration; positive regulation of axon extension; positive regulation of microtubule polymerization; regulation of autophagy; response to nutrient; response to organic substance