Mouse Microtubule Associated Protein Tau (MAPt) ELISA Kit | MAPt elisa kit

Mouse Microtubule Associated Protein Tau (MAPt) ELISA Kit

Cat. Num. AAA1601154

• Gene Names: Mapt; Tau; pTau; Mtapt; RNPTAU
• Reactivity: Mouse
• Synonyms: Microtubule Associated Protein Tau (MAPt); Mouse Microtubule Associated Protein Tau (MAPt) ELISA Kit; MAPt elisa kit

• Reactivity: Mouse
• Sequence Length: 374

• Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 5ng/L - 1900ng/L
• Sensitivity: 2.63ng/L
• Intra-Assay: CV<8%
• Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MAPt elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of Mouse Microtubule Associated Protein Tau (also known as MAPt) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse MAPt antibody. MAPt present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse MAPt Antibody is added and binds to MAPt in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MAPt antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse MAPt. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 158303318
• NCBI GeneID: 29477
• NCBI Accession #: NP_058908.2
• NCBI GenBank Nucleotide #: NM_017212.2
• UniProt Accession #: P19332; Q63567; Q63677; Q9QW06
• Molecular Weight: 71,774 Da
• NCBI Official Full Name: microtubule-associated protein tau
• NCBI Official Synonym Full Names: microtubule-associated protein tau
• NCBI Official Symbol: Mapt
• NCBI Official Synonym Symbols: Tau; pTau; Mtapt; RNPTAU
• NCBI Protein Information: microtubule-associated protein tau
• UniProt Protein Name: Microtubule-associated protein tau
• Protein Family: Microtubule-associated protein
• UniProt Gene Name: Mapt
• UniProt Synonym Gene Names: Mtapt; Tau; PHF-tau

NCBI Description

a microtubule-associated protein; expression is found specifically in neurons [RGD, Feb 2006]

Uniprot Description

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.