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Testing Data

Mammalian OrgFrontier Plasma Membrane Isolation kit

OrgFrontier Plasma Membrane Isolation Kit

Reactivity
Mammalian
Synonyms
OrgFrontier Plasma Membrane; OrgFrontier Plasma Membrane Isolation Kit; OrgFrontier Plasma Membrane isolation kit
Ordering
For Research Use Only!
Reactivity
Mammalian
Samples
Cultured cells and tissues
Features & Benefits
Simple procedure
Fast and convenient
The Membrance Protein Extraction Kit provides an easy and complete protocol for isolating integral membrance proteins efficiently from cultured mammalian cells.
Isolation of plasma membrane fractions from tissues and cultured cells.
Plasma membrane studies and plasma membrane protein profiling.
Enrichment of membrane-associated proteins for western blot and ELISA
Extracts high-quality, intact membrane proteins in less than 2 hours.
Preparation and Storage
Store at -20 degree C
Shelf life: ~12 months

Testing Data

Testing Data

Testing Data

Testing Data
Related Product Information for OrgFrontier Plasma Membrane isolation kit
Plasma membrane is one of the most studied cellular membranes. Prokaryotic and eukaryotic organisms possess plasma membranes which, in summary, enclose and protect the cytosol, and cellular organelles while communicating to their surroundings due to their semi-permeable characteristics. Plasma membranes are selectively permeable lipid bilayers: they allow nutrients and other metabolites to be transported into the cytosol and allow for export of cellular waste at the same time. Oxygen, CO2 and other gaseous or lipophilic small molecules freely diffuse across the plasma membrane; however, ions, amino acids and sugars follow strict passive and active cellular transportation mechanisms. The plasma membrane lipid bilayer is primarily composed of four phospholipids: phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine and sphingomyelin. Phosphatidylinositol, a fifth phospholipid, is also localized in the inner side of the membrane. Additionally, many proteins are embedded or found adhered to both sides of the plasma membrane. For example, glycoproteins are usually found attached to the outer side of the membrane. OrgFrontier Plasma Membrane Isolation Kit provides a proprietary set of reagents and buffers designed to enable optimized extraction and isolation of intact plasma membrane from mammalian tissues and cells. The protocol requires an ultracentrifuge to be performed and can be completed in a few hours.

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Product Notes

The Mammalian OrgFrontier Plasma Membrane (Catalog #AAA849645) is an Isolation kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849645 Isolation kit recognizes Mammalian OrgFrontier Plasma Membrane. It is sometimes possible for the material contained within the vial of "OrgFrontier Plasma Membrane, Isolation kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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