Testing Data
Bacteria Lipopolysaccharide (LPS) Isolation kit
Lipopolysaccharide (LPS) Isolation Kit
Reactivity
Bacteria
Synonyms
Lipopolysaccharide (LPS); Lipopolysaccharide (LPS) Isolation Kit; Lipopolysaccharide (LPS) isolation kit
Reactivity
Bacteria
Samples
A sweep from an overnight culture of bacteria E Coli culture
Features & Benefits
Simple, one step assay to isolate PBMC
This kit does not use chlorofrom or phenol like traditional methods
Yields pure LPS in less than 2 hours that can be easily characterized and quantified
Isolation of LPS from outer membrane of gram negative bacteria.
A sweep from an overnight culture of bacteria grown on an LB plateresuspended in 10 mlof PBSroutinely yieldsan OD of 0.6-1.5. 10 mlof E. coliculture producesapellet approximately 10-100 mgin dry pellet weight with LPS yields 1-4% of dry weight.
This kit does not use chlorofrom or phenol like traditional methods
Yields pure LPS in less than 2 hours that can be easily characterized and quantified
Isolation of LPS from outer membrane of gram negative bacteria.
A sweep from an overnight culture of bacteria grown on an LB plateresuspended in 10 mlof PBSroutinely yieldsan OD of 0.6-1.5. 10 mlof E. coliculture producesapellet approximately 10-100 mgin dry pellet weight with LPS yields 1-4% of dry weight.
Preparation and Storage
Store at -20 degree C
Shelf life: ~12 months
Shelf life: ~12 months
Testing Data
Testing Data
Testing Data
Testing Data
Related Product Information for Lipopolysaccharide (LPS) isolation kit
The outer membrane of gram-negative bacteria contains lipopolysaccharide (LPS), a low molecular weight carbohydrate with a molecular mass of 10-20 kDa. It is heterogeneous, and composed of O antigen (a repeating glycan polymer), core oligosaccharide (which links the O antigen to Lipid A - the third component, and non-carbohydrate components such as phosphate and amino acids groups. Lipid A, has multiple fatty acids which serve to anchor LPS into the bacterial membrane allowing the O antigen and core oligosaccharide to protrude and contributes to a major part of the toxicity of gram-negative bacteria. Also known as endotoxin, when consumed by animals, LPS induces a strong inflammatory response and/or sepsis. LPS Isolation Kit uses bacterial membrane lysis buffer and protein digestion to yield micrograms of LPS from bacterial culture (approximately 1-4% of dry weight). This kit does not use chlorofrom or phenol like traditional methods, and it will yield pure LPS in less than 2 hours that can be easily characterized and quantified!
Product Categories/Family for Lipopolysaccharide (LPS) isolation kit
Similar Products
Product Notes
The Bacteria Lipopolysaccharide (LPS) (Catalog #AAA849661) is an Isolation kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849661 Isolation kit recognizes Bacteria Lipopolysaccharide (LPS). It is sometimes possible for the material contained within the vial of "Lipopolysaccharide (LPS), Isolation kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
