Hepcidin (Hepc) Recombinant Protein | Hepc recombinant protein

Recombinant Hepcidin (Hepc)

Cat. Num. AAA2018431

• Gene Names: Hamp; Hepc
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Hepcidin (Hepc); Recombinant Hepcidin (Hepc); Hepc recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGS- GAYLRQQT RQTTALQPWH GAESKTDDSA LLMLKRRKRD TNFPICLFCC KCCKNSSCGL CCIT
• Sequence Length: 84

• Applicable Applications for Hepc recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Gly23~Thr84 (Accession # Q99MH3) with two N-terminal Tags, His-tag and GST-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 16758218
• NCBI GeneID: 84604
• NCBI Accession #: NP_445921.1
• NCBI GenBank Nucleotide #: NM_053469.1
• UniProt Accession #: Q99MH3
• Molecular Weight: 38.7kDa
• NCBI Official Full Name: hepcidin
• NCBI Official Synonym Full Names: hepcidin antimicrobial peptide
• NCBI Official Symbol: Hamp
• NCBI Official Synonym Symbols: Hepc
• NCBI Protein Information: hepcidin
• UniProt Protein Name: Hepcidin
• Protein Family: Heparin-sulfate lyase
• UniProt Gene Name: Hamp
• UniProt Synonym Gene Names: Hepc
• UniProt Entry Name: HEPC_RAT

NCBI Description

may play a role in the regulation of iron metabolism [RGD, Feb 2006]

Uniprot Description

HAMP: Seems to act as a signaling molecule involved in the maintenance of iron homeostasis. Seems to be required in conjunction with HFE to regulate both intestinal iron absorption and iron storage in macrophages. Defects in HAMP are the cause of hemochromatosis type 2B (HFE2B); also known as juvenile hemochromatosis (JH). HFE2B is a disorder of iron metabolism with excess deposition of iron in the tissues, bronze skin pigmentation, hepatic cirrhosis, arthropathy and diabetes. The most common symptoms of hemochromatosis type 2 at presentation are hypogonadism and cardiomyopathy. Belongs to the hepcidin family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular space; apical cortex; cell; cytoplasm; extracellular region; nucleus

Molecular Function: copper ion binding; hormone activity

Biological Process: iron ion homeostasis; cellular iron ion homeostasis; negative regulation of inflammatory response; myeloid cell homeostasis; defense response to bacterium; positive regulation of transcription from RNA polymerase II promoter; negative regulation of bone resorption; JAK-STAT cascade; defense response to fungus; positive regulation of macrophage activation