Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Leukosialin (SPN) Standard Curve.)
Rat Leukosialin (SPN) ELISA Kit | LSN elisa kit
Rat Leukosialin (SPN) ELISA Kit
Gene Names
Spn; Lsn; Cd43; Lsn1; gpL115
Reactivity
Rat
Synonyms
Leukosialin (SPN); Rat Leukosialin (SPN) ELISA Kit; SPN; CD43; GPL115; leukosialin; sialophorin (gpL115; CD43); sialophorin (leukosialin; LSN elisa kit
Reactivity
Rat
Specificity
Rat Leukosialin (SPN) ELISA Kit has high sensitivity and excellent specificity for detection of Rat SPN. No significant cross-reactivity or interference between Rat SPN and analogues was observed.
Sequence Length
378
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Leukosialin (SPN) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Leukosialin (SPN) Standard Curve.)
Related Product Information for LSN elisa kit
Description: This Rat Leukosialin (SPN) ELISA Kit employs a two-site sandwich ELISA to quantitate SPN in samples. An antibody specific for SPN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Sialophorin (leukosialin) is a major sialoglycoprotein on the surface of human T lymphocytes, monocytes, granulocytes, and some B lymphocytes, which appears to be important for immune function and may be part of a physiologic ligand-receptor complex involved in T-cell activation.The nucleotide sequence of the 1.7-kb clone showed that the sialophorin transcript has a 3-prime-noncoding region of 587 nucleotides, is polyadenylated, and has the AATAAA polyadenylation signal. The derived sequence of 341 amino acids showed that the sialophorin protein has 3 domains: an extracellular domain of 195 amino acids, a hydrophobic transmembrane domain of 23 amino acids, and a C-terminal intracellular domain of 123 amino acids which contains a number of sites that might be phosphorylated by protein kinase C.
Background: Sialophorin (leukosialin) is a major sialoglycoprotein on the surface of human T lymphocytes, monocytes, granulocytes, and some B lymphocytes, which appears to be important for immune function and may be part of a physiologic ligand-receptor complex involved in T-cell activation.The nucleotide sequence of the 1.7-kb clone showed that the sialophorin transcript has a 3-prime-noncoding region of 587 nucleotides, is polyadenylated, and has the AATAAA polyadenylation signal. The derived sequence of 341 amino acids showed that the sialophorin protein has 3 domains: an extracellular domain of 195 amino acids, a hydrophobic transmembrane domain of 23 amino acids, and a C-terminal intracellular domain of 123 amino acids which contains a number of sites that might be phosphorylated by protein kinase C.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Leukosialin
NCBI Official Synonym Full Names
sialophorin
NCBI Official Symbol
Spn
NCBI Official Synonym Symbols
Lsn; Cd43; Lsn1; gpL115
NCBI Protein Information
leukosialin
UniProt Protein Name
Leukosialin
UniProt Gene Name
Spn
UniProt Entry Name
LEUK_RAT
NCBI Description
major glycoprotein of thymocytes and T lymphocytes [RGD, Feb 2006]
Research Articles on LSN
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Product Notes
The Rat LSN spn (Catalog #AAA9711728) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711728 ELISA Kit recognizes Rat LSN. It is sometimes possible for the material contained within the vial of "Leukosialin (SPN), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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