Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Leukosialin (SPN) Standard Curve.)
Mouse Leukosialin (SPN) ELISA Kit | LSN elisa kit
Mouse Leukosialin (SPN) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Leukosialin (SPN) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Leukosialin (SPN) Standard Curve.)
Background: Sialophorin (leukosialin) is a major sialoglycoprotein on the surface of human T lymphocytes, monocytes, granulocytes, and some B lymphocytes, which appears to be important for immune function and may be part of a physiologic ligand-receptor complex involved in T-cell activation.The nucleotide sequence of the 1.7-kb clone showed that the sialophorin transcript has a 3-prime-noncoding region of 587 nucleotides, is polyadenylated, and has the AATAAA polyadenylation signal. The derived sequence of 341 amino acids showed that the sialophorin protein has 3 domains: an extracellular domain of 195 amino acids, a hydrophobic transmembrane domain of 23 amino acids, and a C-terminal intracellular domain of 123 amino acids which contains a number of sites that might be phosphorylated by protein kinase C.
NCBI and Uniprot Product Information
Uniprot Description
sialophorin: One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter-receptor for SN/Siglec-1. During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response. Interacts with HIPK2 via the cytoplasmic domain. Interacts with RDX. Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.
Protein type: Cell surface; Membrane protein, integral
Cellular Component: extracellular space; microvillus; cell surface; membrane; integral to plasma membrane; integral to membrane; basement membrane; uropod; external side of plasma membrane; cleavage furrow
Molecular Function: protein domain specific binding; protein binding
Biological Process: positive regulation of tumor necrosis factor biosynthetic process; regulation of immune response; regulation of defense response to virus; negative regulation of cell adhesion; negative thymic T cell selection; response to protozoan; negative regulation of T cell proliferation; cell surface receptor linked signal transduction; T cell costimulation; defense response to bacterium; positive regulation of T cell proliferation; positive regulation of protein amino acid phosphorylation; negative regulation of type IV hypersensitivity; negative regulation of T cell activation
Research Articles on LSN
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Product Notes
The Mouse LSN spn (Catalog #AAA9716839) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716839 ELISA Kit recognizes Mouse LSN. It is sometimes possible for the material contained within the vial of "Leukosialin (SPN), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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