Mouse anti-Human Leptin Receptor Monoclonal Antibody | anti-LEPR antibody

Leptin Receptor (OBR, LEPR, B219, Obese Receptor)

Cat. Num. AAA606437

• Gene Names: LEPR; OBR; OB-R; CD295; LEP-R; DKFZp686B1731
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Leptin Receptor; Monoclonal Antibody; Leptin Receptor (OBR; LEPR; B219; Obese Receptor); Anti -Leptin Receptor (OBR; anti-LEPR antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 5i316
• Specificity: Recognizes human Leptin Receptor. Does not react with mouse.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a lyophillized powder from 0.05M PBS, pH 7.2. Reconstitute with 100ul sterile ddH2O.

• Applicable Applications for anti-LEPR antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Immunohistochemistry and Western Blot.
• Immunogen: Recombinant corresponding to aa1-839 from human Leptin Receptor expressed in NS0 mouse myeloma cell line.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(Immunohistochemical staining of formalin-fixed, paraffin-embedded human appendix using MBS606437 at 1:40 dilution.)

Immunohistochemistry (IHC)

(Immunohistochemical staining of formalin-fixed, paraffin-embedded human gall bladder using MBS606437 at 1:40 dilution.)

Related Product Information for anti-LEPR antibody

Leptin receptor (OB R) was identified as a leptin binding protein (Leptin, the product of the ob gene, is a single chain 16kD protein consisting of 146aa residues.) OB R was found to be a member of the class I cytokine receptor family with a large extracellular domain comprising 816aa residues. Leptin receptor exists in multiple forms with a common extracellular domain and a variable length cytoplasmatic portion. Alternate splicing from a single gene derives the six isoforms of the Leptin receptor. The soluble form of the Leptin receptor, OB R contains no intracellular motifs or transmembrane residues, thus it consists entirely of the extracellular ligand binding domain of the receptor. Long forms of OB R transcripts were reported to be expressed predominantly in regions of the hypothalamus which provides evidence that Leptin receptor is important in body weight regulation. Expression of short forms of OB-R transcripts have been found in multiple tissues, including the choroid plexus, lung, kidney, and primitive hematopoietic cell populations. Leptin receptor may act as a negative regulator of Leptin activity and it may maintain a pool of available bioactive Leptin by binding and delaying its clearance from circulation. Soluble Leptin receptor levels are indirectly proportional to adiposity and are increased in females versus males. Leptin receptor levels are highest in infants, decrease into adolescence, and remain relatively stable throughout adulthood. Soluble Leptin receptor is also found upregulated in patients with chronic heart failure, end-stage renal disease and anorexia.

Product Categories/Family for anti-LEPR antibody

Antibodies; Abs to Obesity Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 1589772
• NCBI GeneID: 3953
• UniProt Accession #: P48357; Q13592; Q13593; Q13594; Q92919; Q92920; Q92921; Q4G138
• Molecular Weight: 132,494 Da
• NCBI Official Full Name: leptin receptor
• NCBI Official Synonym Full Names: leptin receptor
• NCBI Official Symbol: LEPR
• NCBI Official Synonym Symbols: OBR; OB-R; CD295; LEP-R; DKFZp686B1731
• NCBI Protein Information: leptin receptor; huB219; OB receptor; OTTHUMP00000010692; OTTHUMP00000010693; OTTHUMP00000010694; OTTHUMP00000010695
• UniProt Protein Name: Leptin receptor
• Protein Family: Leptin receptor
• UniProt Gene Name: LEPR
• UniProt Synonym Gene Names: DB; OBR
• UniProt Entry Name: LEPR_HUMAN

NCBI Description

The protein encoded by this gene belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this gene have been associated with obesity and pituitary dysfunction. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. It is noteworthy that this gene and LEPROT gene (GeneID:54741) share the same promoter and the first 2 exons, however, encode distinct proteins (PMID:9207021).

Uniprot Description

LEPR: the receptor for leptin (obesity factor). A single-transmembrane-domain receptor of the cytokine receptor family. On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Five alternatively spliced transcript variants have been reported.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 1p31

Cellular Component: extracellular region; integral to membrane; plasma membrane; receptor complex

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; identical protein binding; protein binding; transmembrane receptor activity

Biological Process: cholesterol metabolic process; cell surface receptor linked signal transduction; negative regulation of gluconeogenesis; cytokine and chemokine mediated signaling pathway; negative regulation of hydrolase activity; multicellular organismal development; energy reserve metabolic process

Disease: Leptin Receptor Deficiency