Lactate Dehydrogenase B (LDHB) Recombinant Protein | LDHB recombinant protein

Recombinant Lactate Dehydrogenase B (LDHB)

Cat. Num. AAA2010285

• Gene Names: Ldhb; Ldh2; H-Ldh; LDH-B; LDH-H; Ldh-2; AI790582
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Lactate Dehydrogenase B (LDHB); Recombinant Lactate Dehydrogenase B (LDHB); LDHB recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-MATLKEKLIA SVADDEAAVP NNKITVVGVG QVGMACAISI LGKSLADELA LVDVLEDKLK GEMMDLQHGS LFLQTPKIVA DKDYSVTANS KIVVVTAGVR QQEGESRLNL VQRNVNVFKF IIPQIVKYSP DCTIIVVSNP VDILTYVTWK LSGLPKHRVI GSGCNLDSAR FRYLMAEKLG IHPSSCHGWI LGEHGDSSVA VWSGVNVAGV SLQELNPEMG TDNDSENWKE VHKMVVDSAY EVIKLKGYTN WAIGLSVADL IESMLKNLSR IHPVSTMVKG MYGIENEVFL SLPCILNARG LTSVINQKLK DDEVAQLRKS ADTLWDIQKD LKDL
• Sequence Length: 334

• Applicable Applications for LDHB recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Met1~Leu334 (Accession # P16125) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6678674
• NCBI GeneID: 16832
• NCBI Accession #: NP_032518.1
• NCBI GenBank Nucleotide #: NM_008492.2
• UniProt Accession #: P16125; Q545Y4
• Molecular Weight: 38.1kDa
• NCBI Official Full Name: L-lactate dehydrogenase B chain
• NCBI Official Synonym Full Names: lactate dehydrogenase B
• NCBI Official Symbol: Ldhb
• NCBI Official Synonym Symbols: Ldh2; H-Ldh; LDH-B; LDH-H; Ldh-2; AI790582
• NCBI Protein Information: L-lactate dehydrogenase B chain; LDH heart subunit; lactate dehydrogenase-B; lactate dehydrogenase 2, B chain
• UniProt Protein Name: L-lactate dehydrogenase B chain
• Protein Family: L-lactate dehydrogenase
• UniProt Gene Name: Ldhb
• UniProt Synonym Gene Names: Ldh-2; Ldh2; LDH-B; LDH-H
• UniProt Entry Name: LDHB_MOUSE

NCBI Description

This gene encodes a subunit of the lactate dehydrogenase enzyme, which catalyzes the reversible conversion of lactate to pyruvate in the glycolytic pathway. This protein is one of two lactate dehydrogenase subunits that combine to form five distinct isozymes with different kinetic parameters. Pseudogenes have been identified on chromosomes 1 and 19. Alternative splicing of this gene results in multiple transcript variants that encode multiple isoforms. [provided by RefSeq, Nov 2014]

Uniprot Description

LDH-B: an enzyme that participates in the final step of anaerobic glycolysis. Catalyzes the conversion of (S)-lactate + NAD+ into pyruvate + NADH.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - propanoate; Amino Acid Metabolism - cysteine and methionine; Oxidoreductase; EC 1.1.1.27; Carbohydrate Metabolism - pyruvate

Cellular Component: mitochondrion; membrane; cytoplasm; myelin sheath

Molecular Function: identical protein binding; lactate dehydrogenase activity; oxidoreductase activity; NAD binding; catalytic activity; L-lactate dehydrogenase activity; kinase binding; oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor

Biological Process: NAD metabolic process; carbohydrate metabolic process; lactate metabolic process; carboxylic acid metabolic process; lactate biosynthetic process from pyruvate