Rabbit anti-Human Lactate Dehydrogenase B (LDHB) Polyclonal Antibody | anti-LDHB antibody

Polyclonal Antibody to Lactate Dehydrogenase B (LDHB)

Cat. Num. AAA2001436

• Gene Names: LDHB; LDH-B; LDH-H; LDHBD; TRG-5; HEL-S-281
• Reactivity: Human
• Applications: Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
• Purity: Affinity Chromatography
• Synonyms: Lactate Dehydrogenase B (LDHB); Polyclonal Antibody; Polyclonal Antibody to Lactate Dehydrogenase B (LDHB); anti-LDHB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against LDHB. It has been selected for its ability to recognize LDHB in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.; MGHHHHHHSGSEF- MATLKEKLIA PVAEEEATVP NNKITVVGVG QVGMACAISI LGKSLADELA LVDVLEDKLK GEMMDLQHGS LFLQTPKIVA DKDYSVTANS KIVVVTAGVR QQEGESRLNL VQRNVNVFKF IIPQIVKYSP DCIIIVVSNP VDILTYVTWK LSGLPKHRVI GSGCNLDSAR FRYLMAEKLG IHPSSCHGWI LGEHGDSSVA VWSGVNVAGV SLQELNPEMG TDNDSENWKE VHKMVVESAY EVIKLKGYTN WAIGLSVADL IESMLKNLSR IHPVSTMVKG MYGIENEVFL SLPCILNARG LTSVINQKLK DDEVAQLKKS ADTLWDIQKD LKDL
• Sequence Length: 334

• Applicable Applications for anti-LDHB antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Western blotting: 1:100-400; Immunocytochemistry in formalin fixed cells: 1:100-500; Immunohistochemistry in formalin fixed frozen section: 1:100-500; Immunohistochemistry in paraffin section: 1:50-200; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunogen: Recombinant LDHB (Met1~Leu334) expressed in E.coli.
• Cross Reactivity: Human, Mouse, Rat
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2058044
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample. Lane1: Human Jurkat Cells; Lane2: Human A431 Cells; Lane3: Mouse Testis Tissue; Lane4: Mouse Kidney Tissue; Lane5: Human Liver Tissue.)

Western Blot (WB)

(Western Blot: Sample: Recombinant LDHB, Human.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P. Samples: Human Tissue))

Knockout Validation

(Knockout Validation: Lane 1: Wild-type Jurkat cell lysate;;Lane 2: LDHB knockout Jurkat cell lysate;;Predicted MW: 37kDa ;Observed MW: 35kDa;Primary Ab: 1ug/ml Rabbit Anti-Human LDHB Ab;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;(#MBS2086047))

Product Categories/Family for anti-LDHB antibody

Knock-Out Validation Antibody

NCBI and Uniprot Product Information

• NCBI GI #: 291575128
• NCBI GeneID: 3945
• NCBI Accession #: NP_001167568.1
• NCBI GenBank Nucleotide #: NM_001174097.2
• UniProt Accession #: P07195
• Molecular Weight: 36,638 Da
• NCBI Official Full Name: L-lactate dehydrogenase B chain isoform LDHB
• NCBI Official Synonym Full Names: lactate dehydrogenase B
• NCBI Official Symbol: LDHB
• NCBI Official Synonym Symbols: LDH-B; LDH-H; LDHBD; TRG-5; HEL-S-281
• NCBI Protein Information: L-lactate dehydrogenase B chain
• UniProt Protein Name: L-lactate dehydrogenase B chain
• Protein Family: L-lactate dehydrogenase
• UniProt Gene Name: LDHB
• UniProt Synonym Gene Names: LDH-B; LDH-H

NCBI Description

This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on chromosomes X, 5 and 13. [provided by RefSeq, Feb 2016]