SDS-Page
(Figure. SDS-PAGE)
Superoxide Dismutase 3, Extracellular Active Protein | SOD3 active protein
Active Superoxide Dismutase 3, Extracellular (SOD3)
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
SDS-Page
(Figure. SDS-PAGE)
SDS-Page
(Figure. SDS-PAGE)
Testing Data
(Extracellular superoxide dismutase [Cu-Zn] is an enzyme that in humans is encoded by the SOD3 gene. This gene encodes a member of the superoxide dismutase (SOD) protein family. SODs are antioxidant enzymes that catalyze the dismutation of two superoxide radicals into hydrogen peroxide and oxygen. Acroding to the report, in a weakly alkaline buffer solution (pH=8.2) with N-tris (hydroxymethyl) amino methane-HCL, pyrogallol can occur autoxidation in the air, then SOD can inhibit this reaction. Thus, we use this way to measure the activity of recombinant human SOD3. The reaction was performed in adding 30L 50mmol/L pyrogallol to 900L 50mmol/L Tris-HCl in 1.5ml cuvette (1.0cm light path), rapidly mixing at 25,then read at 325nm (zero the spectrophotometer using 50mmol/L Tris-HCl), reacod the OD value every 30 second for 6 times. Control the pyrogallol autoxidation rate at 0.70 OD/min. After, adding various concentrations of recombinant of SOD3 to 900L 50mmol/L Tris-HCl, incubated for 20min at 25, then adding 30L 50mmol/L pyrogallol to each tube, rapidly mixing and read at 325nm, record the OD value every 30 second for 6 times. Under these conditions, the enzyme amount of 50% per minute inhibition of pyrogallol autooxidation is defined as a unit.)
Testing Data
(Extracellular superoxide dismutase [Cu-Zn] is an enzyme that in humans is encoded by the SOD3 gene. This gene encodes a member of the superoxide dismutase (SOD) protein family. SODs are antioxidant enzymes that catalyze the dismutation of two superoxide radicals into hydrogen peroxide and oxygen. Acroding to the report, in a weakly alkaline buffer solution (pH=8.2) with N-tris (hydroxymethyl) amino methane-HCL, pyrogallol can occur autoxidation in the air, then SOD can inhibit this reaction. Thus, we use this way to measure the activity of recombinant human SOD3. The reaction was performed in adding 30L 50mmol/L pyrogallol to 900L 50mmol/L Tris-HCl in 1.5ml cuvette (1.0cm light path), rapidly mixing at 25,then read at 325nm (zero the spectrophotometer using 50mmol/L Tris-HCl), reacod the OD value every 30 second for 6 times. Control the pyrogallol autoxidation rate at 0.70 OD/min. After, adding various concentrations of recombinant of SOD3 to 900L 50mmol/L Tris-HCl, incubated for 20min at 25, then adding 30L 50mmol/L pyrogallol to each tube, rapidly mixing and read at 325nm, record the OD value every 30 second for 6 times. Under these conditions, the enzyme amount of 50% per minute inhibition of pyrogallol autooxidation is defined as a unit.)
NCBI and Uniprot Product Information
Accurate Molecular Mass: 28kDa
NCBI Description
This gene encodes a member of the superoxide dismutase (SOD) protein family. SODs are antioxidant enzymes that catalyze the conversion of superoxide radicals into hydrogen peroxide and oxygen, which may protect the brain, lungs, and other tissues from oxidative stress. Proteolytic processing of the encoded protein results in the formation of two distinct homotetramers that differ in their ability to interact with the extracellular matrix (ECM). Homotetramers consisting of the intact protein, or type C subunit, exhibit high affinity for heparin and are anchored to the ECM. Homotetramers consisting of a proteolytically cleaved form of the protein, or type A subunit, exhibit low affinity for heparin and do not interact with the ECM. A mutation in this gene may be associated with increased heart disease risk. [provided by RefSeq, Oct 2015]
Uniprot Description
SOD3: Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen. Belongs to the Cu-Zn superoxide dismutase family.
Protein type: EC 1.15.1.1; Secreted, signal peptide; Oxidoreductase; Secreted
Chromosomal Location of Human Ortholog: 4p15.2
Cellular Component: extracellular matrix; extracellular space; Golgi lumen; cytoplasm; extracellular region; trans-Golgi network; nucleus
Molecular Function: heparin binding; protein binding; copper ion binding; zinc ion binding; superoxide dismutase activity
Biological Process: response to reactive oxygen species; response to copper ion; removal of superoxide radicals; response to hypoxia
Research Articles on SOD3
Similar Products
Product Notes
The SOD3 sod3 (Catalog #AAA2122285) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Trp19~Ala240. AAA Biotech's Superoxide Dismutase 3, Extracellular can be used in a range of immunoassay formats including, but not limited to, Cell Culture, Activity Assays. Researchers should empirically determine the suitability of the SOD3 sod3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Superoxide Dismutase 3, Extracellular, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
