LDHB active protein

LDHB, 1-334aa, Human, E Coli (Bioactivity Validated)

Cat. Num. AAA206393

• Gene Names: LDHB; LDH-B; LDH-H; LDHBD; TRG-5; HEL-S-281
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: LDHB; 1-334aa; Human; E Coli (Bioactivity Validated); Lactate dehydrogenase B; HEL-S-281; LDH-B; LDH-H; LDHBD; TRG-5; Epididymis secretory protein Li 281; HEL S 281; L lactate dehydrogenase B chain; L-lactate dehydrogenase B chain; Lactate dehydrogenase H chain; LDH B; LDH H; LDH heart subunit; LDHB_HUMAN; LDHH; Renal carcinoma antigen NY REN 46; Renal carcinoma antigen NY-REN-46; TRG5; LDHB active protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl (pH 8.0) containing 10% glycerol, 1mM DTT
• Concentration: 1mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MATLKEKLIA PVAEEEATVP NNKITVVGVG QVGMACAISI LGKSLADELA LVDVLEDKLK GEMMDLQHGS LFLQTPKIVA DKDYSVTANS KIVVVTAGVR QQEGESRLNL VQRNVNVFKF IIPQIVKYSP DCIIIVVSNP VDILTYVTWK LSGLPKHRVI GSGCNLDSAR FRYLMAEKLG IHPSSCHGWI LGEHGDSSVA VWSGVNVAGV SLQELNPEMG TDNDSENWKE VHKMVVESAY EVIKLKGYTN WAIGLSVADL IESMLKNLSR IHPVSTMVKG MYGIENEVFL SLPCILNARG LTSVINQKLK DDEVAQLKKS ADTLWDIQKD LKDL
• Sequence Length: 334

• Applicable Applications for LDHB active protein: SDS-PAGE
• Antigen Species: Human
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Biological Activity: Specific activity is > 300 units/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for LDHB active protein

LDHB, also known as L-lactate dehydrogenase B chain isoform LDHB, is a member of the lactate dehydrogenase family. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As this protein can also catalyze the oxidation of hydroxybutyrate. The LDH family consists of three members, LDH-A, LDH-B and LDH-C. LDHs function as powerful markers for germ cell tumors. Recombinant human LDHB protein was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for LDHB active protein

Cancer; Metabolism

References

Zhao Z., et al. (2015) Cancer Lett. 358 (1):17-26.; Martin I., et al. (2014) Cell. 157 (2):472-85.

NCBI and Uniprot Product Information

• NCBI GI #: 4557032
• NCBI GeneID: 3945
• NCBI Accession #: NP_002291
• NCBI GenBank Nucleotide #: NM_002300.7
• UniProt Accession #: P07195
• Molecular Weight: 36.6kDa (334aa) confirmed by MALDI-TOF
• NCBI Official Full Name: L-lactate dehydrogenase B chain isoform LDHB
• NCBI Official Synonym Full Names: lactate dehydrogenase B
• NCBI Official Symbol: LDHB
• NCBI Official Synonym Symbols: LDH-B; LDH-H; LDHBD; TRG-5; HEL-S-281
• NCBI Protein Information: L-lactate dehydrogenase B chain
• UniProt Protein Name: L-lactate dehydrogenase B chain
• Protein Family: L-lactate dehydrogenase
• UniProt Gene Name: LDHB
• UniProt Synonym Gene Names: LDH-B; LDH-H

NCBI Description

This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on chromosomes X, 5 and 13. [provided by RefSeq, Feb 2016]