Human Rap1 GTPase-GDP dissociation stimulator 1 ELISA Kit | RAP1GDS1 elisa kit

Human Rap1 GTPase-GDP dissociation stimulator 1 ELISA Kit

Cat. Num. AAA764430

• Gene Names: RAP1GDS1; GDS1; SmgGDS
• Reactivity: Human
• Synonyms: Rap1 GTPase-GDP dissociation stimulator 1; Human Rap1 GTPase-GDP dissociation stimulator 1 ELISA Kit; RAP1GDS1/Rap1 GTPase-GDP dissociation stimulator 1/SMG P21 stimulatory GDP/GTP exchange protein/Exchange factor smgGDS/SMG GDS protein; RAP1GDS1 elisa kit

• Reactivity: Human
• Sequence Length: 608

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for RAP1GDS1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human RAP1GDS1antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human RAP1GDS1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human RAP1GDS1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofHuman RAP1GDS1can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 155030192
• NCBI GeneID: 5910
• NCBI Accession #: NP_001093896.1
• NCBI GenBank Nucleotide #: NM_001100426.1
• Molecular Weight: 56,536 Da
• NCBI Official Full Name: rap1 GTPase-GDP dissociation stimulator 1 isoform 1
• NCBI Official Synonym Full Names: RAP1, GTP-GDP dissociation stimulator 1
• NCBI Official Symbol: RAP1GDS1
• NCBI Official Synonym Symbols: GDS1; SmgGDS
• NCBI Protein Information: rap1 GTPase-GDP dissociation stimulator 1
• UniProt Protein Name: Rap1 GTPase-GDP dissociation stimulator 1
• Protein Family: Rap1 GTPase-GDP dissociation stimulator
• UniProt Gene Name: RAP1GDS1
• UniProt Entry Name: GDS1_HUMAN

NCBI Description

The smg GDP dissociation stimulator (smgGDS) protein is a stimulatory GDP/GTP exchange protein with GTPase activity (Riess et al., 1993 [PubMed 8262526]).[supplied by OMIM, Feb 2010]

Uniprot Description

RAP1GDS1: Stimulates GDP/GTP exchange reaction of a group of small GTP-binding proteins (G proteins) including Rap1a/Rap1b, RhoA, RhoB and KRas, by stimulating the dissociation of GDP from and the subsequent binding of GTP to each small G protein. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs, misc.; GEFs

Chromosomal Location of Human Ortholog: 4q23-q25

Cellular Component: cytosol; endoplasmic reticulum; mitochondrion

Molecular Function: GTPase activator activity; protein binding

Biological Process: elevation of mitochondrial calcium ion concentration; positive regulation of GTPase activity; reduction of endoplasmic reticulum calcium ion concentration; small GTPase mediated signal transduction