SRY recombinant protein

Bovine SRY

Cat. Num. AAA692130

• Reactivity: Bovine
• Purity: > 90% by SDS-PAGE & silver stain
• Synonyms: SRY; Bovine SRY; Recombinant Bovine SRY; Sex-determining region Y protein; Testis-determining factor; SRY recombinant protein

• Host: E Coli
• Reactivity: Bovine
• Purity/Purification: > 90% by SDS-PAGE & silver stain
• Form/Format: Lyophilized
• Sequence: N-Terminal Sequence: MFRVLN
• Sequence Length: 229

• N Terminal Sequence: MFRVLN
• Buffer: ddH2O
• Preparation and Storage: The lyophilized powder although stable at room temperature for 3 weeks, is best stored desiccated at -20 degree C. Reconstituted bo SRY should be stored in working aliquots at -20 degree C. For long term storage it is recommended to add a carrier protein (0.1% HAS or BSA).

Testing Data

Related Product Information for SRY recombinant protein

The "žSex determining region of Y-Gen" (SRY), known as SRY gene, is coding for a transkription factor (TDF for Testis-determining factor). It belongs to the protein family of DNA binding-proteins. The SRY gene is beside other genes used for the determination of the sex in human as well as other mammals. Most mammals have for this purpose a further gene, UBE1. In humans SRY is normally located on the short arm of the Y chromosome. Accordingly, people who possess this chromosome with the corresponding gene, normally have a male phenotype. It does not matter how many copies of the X chromosome are present, even people with a multiple X chromosome (Klinefelter\'s syndrome) have this. The protein encoded by the gene Testis-determining factor controls the development of male sex

Product Categories/Family for SRY recombinant protein

Cytokines & Growth Factors

References

1. Harikae K et al, J Reprod Dev 2012; 58:654-60 2. Hamilton CK et al, Theriogenology 2012; 77:1587-96 3. Gou X et al, J Anim Breed Genet 2010; 127:154-60 4. Ross DG et al, BMC Mol Biol 2008; 9:85 5. Lu W et al, Anim Reprod Sci 2007; 100:186-91

NCBI and Uniprot Product Information

• NCBI GI #: 62177084
• NCBI GeneID: 280931
• NCBI Accession #: NP_001014407.1
• NCBI GenBank Nucleotide #: NM_001014385.1
• UniProt Accession #: Q03255; A1XCJ6; A8WDV8
• Molecular Weight: 26,8 kDa
• NCBI Official Full Name: sex-determining region Y protein
• NCBI Official Symbol: SRY
• NCBI Protein Information: sex-determining region Y protein; sex determining factor Y; testis-determining factor; sex-determining region of the Y-chromosome
• UniProt Protein Name: Sex-determining region Y protein
• Protein Family: SRY-related protein
• UniProt Gene Name: SRY
• UniProt Synonym Gene Names: TDF
• UniProt Entry Name: SRY_BOVIN

Uniprot Description

Function: Transcriptional regulator that controls a genetic switch in male development. It is necessary and sufficient for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells. In male adult brain involved in the maintenance of motor functions of dopaminergic neurons. Involved in different aspects of gene regulation including promoter activation or repression. Promotes DNA bending. SRY HMG box recognizes DNA by partial intercalation in the minor groove. Also involved in pre-mRNA splicing. Binds to the DNA consensus sequence 5'-[AT]AACAA[AT]-3'

By similarity.

Subunit structure: Interacts with CALM, EP300, HDAC3, KPNB1, KRAB-O/ZFP748, PARP1, SLC9A3R2 and WT1

By similarity.

Subcellular location: Nucleus speckle

By similarity. Cytoplasm

By similarity.

Post-translational modification: Phosphorylation on serine residues enhances its DNA-binding activity and stimulates transcription repression. Acetylation of Lys-130 contributes to its nuclear localization and enhances its interaction with KPNB1. Deacetylated by HDAC3. Poly-ADP-ribosylation reduces its DNA-binding activity

By similarity.

Sequence similarities: Belongs to the SRY family.Contains 1 HMG box DNA-binding domain.