Rabbit anti-Human Hexokinase 2 Polyclonal Antibody | anti-HXK2 antibody

Hexokinase 2 (Hexokinase-2, Hexokinase Type II, HK II, HKII, HK2, HXK2, DKFZp686M1669, Muscle Form Hexokinase) (Biotin)

Cat. Num. AAA6480197

• Gene Names: HK2; HKII; HXK2
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Hexokinase 2; Polyclonal Antibody; Hexokinase 2 (Hexokinase-2; Hexokinase Type II; HK II; HKII; HK2; HXK2; DKFZp686M1669; Muscle Form Hexokinase) (Biotin); EC=2.7.1.1; anti-HXK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HK2.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-HXK2 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human HK2 (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-HK2 Pab is used in Western blot to detect HK2 in A375 cell lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-HXK2 antibody

Antibodies; Enzymes

References

1. Lehto, M., et al., Diabetologia 38(12):1466-1474 (1995). 2. Vidal-Puig, A., et al., Diabetes 44(3):340-346 (1995). 3. Laakso, M., et al., Diabetes 44(3):330-334 (1995). 4. Echwald, S.M., et al., Diabetes 44(3):347-353 (1995). 5. Shinohara, Y., et al., Cancer Lett. 82(1):27-32 (1994). 6.Diane Palmieri, et al. Mol. Cancer Res. 2009; 7:1438-1445.

NCBI and Uniprot Product Information

• NCBI GI #: 39963174
• NCBI GeneID: 3099
• UniProt Accession #: P52789; Q8WU87; Q9UN82; D6W5J2
• Molecular Weight: 102,380 Da
• NCBI Official Full Name: Hexokinase 2
• NCBI Official Synonym Full Names: hexokinase 2
• NCBI Official Symbol: HK2
• NCBI Official Synonym Symbols: HKII; HXK2
• NCBI Protein Information: hexokinase-2; HK II; hexokinase type II; hexokinase-2, muscle; muscle form hexokinase
• UniProt Protein Name: Hexokinase-2
• Protein Family: Hexokinase
• UniProt Gene Name: HK2
• UniProt Synonym Gene Names: HK II
• UniProt Entry Name: HXK2_HUMAN

NCBI Description

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq, Apr 2009]

Uniprot Description

HK2: a glycolytic enzyme that catalyzes the reaction ATP + D-hexose = ADP + D-hexose 6-phosphate. The first and rate-limiting step in glycosis, a pathway that produces energy in the form of ATP from glucose. An allosteric enzyme inhibited by its product glucose-6-phosphate (Glc-6-P). The predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle. Acts as a ""glucose sensor"" by trapping glucose inside the cell by catalyzing its phosphorylation to produce Glc-6-P. In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase). Genetic variations of HK2 do not appear to contribute to NIDDM.

Protein type: Kinase, other; Carbohydrate Metabolism - starch and sucrose; EC 2.7.1.1; Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - galactose; Mitochondrial; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Carbohydrate Metabolism - glycolysis and gluconeogenesis

Chromosomal Location of Human Ortholog: 2p13

Cellular Component: mitochondrial outer membrane; membrane; cytosol

Molecular Function: hexokinase activity; mannokinase activity; fructokinase activity; glucokinase activity; ATP binding; glucose binding

Biological Process: lactation; apoptotic mitochondrial changes; glycolysis; hexose transport; glucose 6-phosphate metabolic process; carbohydrate metabolic process; carbohydrate phosphorylation; glucose metabolic process; cell glucose homeostasis; regulation of glucose import; pathogenesis; glucose transport; transmembrane transport