Lymphocyte Activation Gene 3 (LAG3) Recombinant Protein | LAG3 recombinant protein

Recombinant Lymphocyte Activation Gene 3 (LAG3)

Cat. Num. AAA2011091

• Gene Names: Lag3; Ly66; CD223; LAG-3
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Lymphocyte Activation Gene 3 (LAG3); Recombinant Lymphocyte Activation Gene 3 (LAG3); LAG3 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-LPCHLPP GVGTPSLLIA KWTPPGGGPE LPVAGKSGNF TLHLEAVGLA QAGTYTCSIH LQGQQLNATV TLAVITVTPK SFGLPGSRGK LLCEVTPASG KERFVWRPLN NLSRSCPGPV LEIQEARLLA ERWQCQLYEG QRLLGATVYA AESSSGAHSA RRISGDLKGG HLVLVLILGA LSLFLLVAGA FGFHWWRKQL LLRRFSALEH GIQPFPAQRK IEELERELET EMGQ
• Sequence Length: 521

• Applicable Applications for LAG3 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Leu274~Gln504 (Accession # Q61790) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6678654
• NCBI GeneID: 16768
• NCBI Accession #: NP_032505.1
• NCBI GenBank Nucleotide #: NM_008479.2
• UniProt Accession #: Q61790; Q0VBL2
• Molecular Weight: 28.8kDa
• NCBI Official Full Name: lymphocyte activation gene 3 protein
• NCBI Official Synonym Full Names: lymphocyte-activation gene 3
• NCBI Official Symbol: Lag3
• NCBI Official Synonym Symbols: Ly66; CD223; LAG-3
• NCBI Protein Information: lymphocyte activation gene 3 protein
• UniProt Protein Name: Lymphocyte activation gene 3 protein
• Protein Family: Lymphocyte activation gene 3 protein
• UniProt Gene Name: Lag3
• UniProt Synonym Gene Names: LAG-3
• UniProt Entry Name: LAG3_MOUSE

Uniprot Description

LAG3: Involved in lymphocyte activation. Binds to HLA class-II antigens.

Protein type: Membrane protein, integral

Cellular Component: membrane; integral to membrane; external side of plasma membrane

Molecular Function: transmembrane receptor activity; MHC class II protein binding

Biological Process: cell surface receptor linked signal transduction; negative regulation of interleukin-2 biosynthetic process; positive regulation of natural killer cell mediated cytotoxicity; negative regulation of T cell activation