Mouse lymphocyte-activation gene 3 ELISA Kit | LAG3 elisa kit

Mouse Lymphocyte activation gene 3 protein, LAG3 ELISA Kit

Cat. Num. AAA934816

• Gene Names: Lag3; Ly66; CD223; LAG-3
• Reactivity: Mouse
• Synonyms: lymphocyte-activation gene 3; Mouse Lymphocyte activation gene 3 protein; LAG3 ELISA Kit; Mouse Lymphocyte activation gene 3 protein (LAG3) ELISA kit; CD223; lymphocyte-activation protein 3; LAG3 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse LAG3. No significant cross-reactivity or interference between mouse LAG3 and analogues was observed.
• Sequence Length: 521

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 1.25 ng/ml-80 ng/ml
• Sensitivity: < 0.31 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LAG3 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LAG3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LAG3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LAG3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LAG3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 6678654
• NCBI GeneID: 16768
• NCBI Accession #: NP_032505.1
• NCBI GenBank Nucleotide #: NM_008479.2
• UniProt Accession #: Q61790; Q0VBL2
• Molecular Weight: 56,978 Da
• NCBI Official Full Name: lymphocyte activation gene 3 protein
• NCBI Official Synonym Full Names: lymphocyte-activation gene 3
• NCBI Official Symbol: Lag3
• NCBI Official Synonym Symbols: Ly66; CD223; LAG-3
• NCBI Protein Information: lymphocyte activation gene 3 protein
• UniProt Protein Name: Lymphocyte activation gene 3 protein
• Protein Family: Lymphocyte activation gene 3 protein
• UniProt Gene Name: Lag3
• UniProt Synonym Gene Names: LAG-3
• UniProt Entry Name: LAG3_MOUSE

Uniprot Description

LAG3: Involved in lymphocyte activation. Binds to HLA class-II antigens.

Protein type: Membrane protein, integral

Cellular Component: membrane; integral to membrane; external side of plasma membrane

Molecular Function: transmembrane receptor activity; MHC class II protein binding

Biological Process: cell surface receptor linked signal transduction; negative regulation of interleukin-2 biosynthetic process; positive regulation of natural killer cell mediated cytotoxicity; negative regulation of T cell activation