Rat CD223 Monoclonal Antibody | anti-CD223 antibody

RAT ANTI MOUSE CD223:Low Endotoxin

Cat. Num. AAA211241

• Gene Names: Lag3; Ly66; CD223; LAG-3
• Applications: Flow Cytometry, Functional Assay, Functional Assay
• Synonyms: CD223; Monoclonal Antibody; RAT ANTI MOUSE CD223:Low Endotoxin; anti-CD223 antibody

• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: C9B7W
• Form/Format: Low Endotoxin; Purified IgG - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 521

• Applicable Applications for anti-CD223 antibody: Flow cytometry (FC/FACS), Functional Assays (FN)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. ; Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/200
• Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Immunogen: Murine CD223 Ig fusion protein; Fusion Partners
• Target Species: Mouse
• Preparation and Storage: Store at -20 degree C only. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data

(Staining of CD223 transfected cells with Rat anti Mouse CD223 (MBS212757))

Testing Data

(Staining of CD223 transfected cells with Rat anti Mouse CD223:Alexa Fluor 647 (MBS212756A647))

Testing Data

(Staining of CD223 transfected cells with Rat anti Mouse CD223:Alexa Fluor 488 (MBS212756A488))

Testing Data

(Staining of CD223 transfected cells with Rat anti Mouse CD223 (MBS212756))

Testing Data

(Staining of CD223 transfected cells with Rat anti Mouse CD223:RPE (MBS216008))

Related Product Information for anti-CD223 antibody

Rat anti Mouse CD223 antibody, clone C9B7W recognizes murine lymphocyte activation gene-3 (LAG-3), a 70kD activation-induced cell surface molecule that is also referred to as CD223. Murine CD223 is expressed on activated CD4 positive and CD8 positive alpha/beta T lymphocytes and a subset of natural killer (NK) cells. CD223 binds to MHC class II molecules with high affinity and is reported to negatively regulate T cell homeostasis and T cell expansion. Clone C9B7W recognizes an epitope within the D2 domain of CD223. The antibody is reported to block the in vitro function of murine LAG-3 but studies suggest that the antibody does not block binding of LAG-3 to MHC class II (Workman et al. 2002).

NCBI and Uniprot Product Information

• NCBI GI #: 6678654
• NCBI GeneID: 16768
• NCBI Accession #: NP_032505.1
• NCBI GenBank Nucleotide #: NM_008479.2
• UniProt Accession #: Q61790; Q0VBL2
• Molecular Weight: 56,978 Da
• NCBI Official Full Name: lymphocyte activation gene 3 protein
• NCBI Official Synonym Full Names: lymphocyte-activation gene 3
• NCBI Official Symbol: Lag3
• NCBI Official Synonym Symbols: Ly66; CD223; LAG-3
• NCBI Protein Information: lymphocyte activation gene 3 protein
• UniProt Protein Name: Lymphocyte activation gene 3 protein
• UniProt Gene Name: Lag3
• UniProt Synonym Gene Names: LAG-3
• UniProt Entry Name: LAG3_MOUSE

Uniprot Description

LAG3: Involved in lymphocyte activation. Binds to HLA class-II antigens.

Protein type: Membrane protein, integral

Cellular Component: membrane; integral to membrane; external side of plasma membrane

Molecular Function: transmembrane receptor activity; MHC class II protein binding

Biological Process: cell surface receptor linked signal transduction; negative regulation of interleukin-2 biosynthetic process; positive regulation of natural killer cell mediated cytotoxicity; negative regulation of T cell activation