VWC2 / Brorin Recombinant Protein | VWC2 recombinant protein

Recombinant Human VWC2 / Brorin Protein (His tag)

Cat. Num. AAA2545587

• Gene Names: NLGN1; NL1
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: VWC2 / Brorin; Recombinant Human VWC2 / Brorin Protein (His tag); UNQ739/PRO1434; MGC131845; PSST739; UNQ739; VWC2 recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 823

• Application Notes: The recombinant human VWC2 consists of 309 amino acids and predictes a molecular mass of 34.1 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh VWC2 is approximately 45 kDa due to glycosylation.
• Predicted N Terminal: Ser 28
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for VWC2 recombinant protein

Background: Brorin, also known as brain-specific chordin-like protein, von Willebrand factor C domain-containing protein 2 and VWC2, is a secreted protein which contains two VWFC domains. VWC2 / Brorin is a BMP antagonist which may play a role in neural development. It promotes cell adhesion. VWC2 / Brorin is a unique member of the chordin family. It inhibited the activity of bone morphogenetic protein 2 (BMP2) and BMP6 in mouse preosteoblastic MC3T3-E1 cells. Mouse Brorin was predominantly expressed in neural tissues in embryos and also predominantly expressed in the adult brain. In the brain, the expression was detected in neurons, but not glial cells. The neural tissue-specific expression profile of Brorin is quite distinct from that of any other member of the Chordin family. VWC2 / Brorin protein promoted neurogenesis, but not astrogenesis, in mouse neural precursor cells. VWC2 / Brorin is a novel secreted BMP antagonist that potentially plays roles in neural development and functions.

Description: A DNA sequence encoding the human VWC2 (NP_055747.1) (Met 1-Met 325) was expressed, fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 7662470
• NCBI GeneID: 22871
• NCBI Accession #: NP_055747.1
• NCBI GenBank Nucleotide #: NM_014932.3
• UniProt Accession #: Q8N2Q7; Q9UPT2
• Molecular Weight: 92,000 Da
• NCBI Official Full Name: neuroligin-1
• NCBI Official Synonym Full Names: neuroligin 1
• NCBI Official Symbol: NLGN1
• NCBI Official Synonym Symbols: NL1
• NCBI Protein Information: neuroligin-1
• UniProt Protein Name: Neuroligin-1
• Protein Family: Brorin
• UniProt Gene Name: NLGN1
• UniProt Synonym Gene Names: KIAA1070

NCBI Description

This gene encodes a member of a family of neuronal cell surface proteins. Members of this family may act as splice site-specific ligands for beta-neurexins and may be involved in the formation and remodeling of central nervous system synapses. [provided by RefSeq, Jul 2008]

Uniprot Description

NLGN1: Cell surface protein involved in cell-cell-interactions via its interactions with neurexin family members. Plays a role in synapse function and synaptic signal transmission, and probably mediates its effects by recruiting and clustering other synaptic proteins. May promote the initial formation of synapses, but is not essential for this. In vitro, triggers the de novo formation of presynaptic structures. May be involved in specification of excitatory synapses. Belongs to the type-B carboxylesterase/lipase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q26.31

Cellular Component: cell surface; dendrite; dendritic spine; extracellular space; filopodium tip; Golgi apparatus; integral to plasma membrane; plasma membrane; postsynaptic density; synapse

Molecular Function: cell adhesion molecule binding; neurexin binding; PDZ domain binding; protein dimerization activity; receptor activity

Biological Process: calcium-dependent cell-cell adhesion; cytoskeletal matrix organization at active zone; establishment of protein localization; heterophilic cell adhesion; nervous system development; neurite development; neuron adhesion; positive regulation of filopodium formation; positive regulation of synaptic transmission, GABAergic; positive regulation of synaptic transmission, glutamatergic; positive regulation of synaptogenesis; protein homooligomerization; protein targeting; regulation of neuron differentiation; synaptic vesicle targeting; synaptogenesis