Human Cytokeratin 18 ELISA Kit | CK-18/KRT18 elisa kit

Human Cytokeratin 18 ELISA Kit

Cat. Num. AAA761380

• Gene Names: KRT18; K18; CK-18; CYK18
• Reactivity: Human
• Synonyms: Cytokeratin 18; Human Cytokeratin 18 ELISA Kit; KRT18/CK-18/Cytokeratin 18/Keratin; type I cytoskeletal 18/Keratin-18/K18/Cell proliferation-inducing gene 46 protein/Cytokeratin-18; CK-18/KRT18 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CK-18/KRT18 . No significant cross-reactivity or interference between CK-18/KRT18 and analogues was observed.
• Sequence Length: 430

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: <0.094ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CK- 18/KRT18 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CK- 18/KRT18 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CK-18/KRT18 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- CK- 18/KRT18 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- CK- 18/KRT18 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CK-18/KRT18 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CK- 18/KRT18 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 34037
• NCBI GeneID: 3875
• UniProt Accession #: P05783; Q53G38; Q5U0N8; Q9BW26
• Molecular Weight: 48,058 Da
• NCBI Official Full Name: cytokeratin 18
• NCBI Official Synonym Full Names: keratin 18, type I
• NCBI Official Symbol: KRT18
• NCBI Official Synonym Symbols: K18; CK-18; CYK18
• NCBI Protein Information: keratin, type I cytoskeletal 18
• UniProt Protein Name: Keratin, type I cytoskeletal 18
• UniProt Gene Name: KRT18
• UniProt Synonym Gene Names: CYK18; CK-18; K18
• UniProt Entry Name: K1C18_HUMAN

NCBI Description

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

K18: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Keratin 18 and its filament partner keratin 8 are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Defects are a cause of cryptogenic cirrhosis.

Protein type: Nucleolus; Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: cytoplasm; intermediate filament; keratin filament; microtubule organizing center; nucleolus; perinuclear region of cytoplasm

Molecular Function: protein binding; structural molecule activity

Biological Process: anatomical structure morphogenesis; cell cycle; Golgi to plasma membrane CFTR protein transport; intermediate filament cytoskeleton organization and biogenesis; negative regulation of apoptosis; tumor necrosis factor-mediated signaling pathway; viral reproduction

Disease: Cirrhosis, Familial