Mouse Integrin alpha-V ELISA Kit | Itgav elisa kit

Mouse Integrin alpha-V ELISA Kit

Cat. Num. AAA288043

• Gene Names: Itgav; CD51; 1110004F14Rik; 2610028E01Rik; D430040G12Rik
• Reactivity: Mouse
• Synonyms: Integrin alpha-V; Mouse Integrin alpha-V ELISA Kit; Itgav elisa kit

• Reactivity: Mouse
• Specificity: This assay recognizes recombinant and natural mouse Integrin alpha-V. No significant cross-reactivity or interference was observed.
• Sequence Length: 1012

• Assay Type: Sandwich
• Detection Range: 78-5000 pg/ml
• Sensitivity: 39 pg/mL
• Intra-Assay CV: <=4.7%
• Inter-Assay CV: <=7.1%
• Recovery: 82%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Itgav elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Integrin alpha-V. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Integrin alpha-V and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Integrin alpha-V, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Integrin alpha-V in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 154240716
• NCBI GeneID: 16410
• NCBI Accession #: NP_032428.2
• NCBI GenBank Nucleotide #: NM_008402.3
• UniProt Accession #: P43406; A2AKI6
• Molecular Weight: 115,360 Da
• NCBI Official Full Name: integrin alpha-V
• NCBI Official Synonym Full Names: integrin alpha V
• NCBI Official Symbol: Itgav
• NCBI Official Synonym Symbols: CD51; 1110004F14Rik; 2610028E01Rik; D430040G12Rik
• NCBI Protein Information: integrin alpha-V
• UniProt Protein Name: Integrin alpha-V
• Protein Family: Integrin
• UniProt Gene Name: Itgav
• UniProt Entry Name: ITAV_MOUSE

NCBI Description

This gene encodes a protein that is a member of the integrin superfamily. Integrins are transmembrane receptors involved cell adhesion and signaling, and they are subdivided based on the heterodimer formation of alpha and beta chains. This protein has been shown to heterodimerize with beta 1, beta 3, beta 6 and beta 8. The heterodimer of alpha v and beta 3 forms the Vitronectin receptor. This protein interacts with several extracellular matrix proteins to mediate cell adhesion and may play a role in cell migration. In mouse, deficiency of this gene is associated with defects in vascular morphogenesis in the brain and early post-natal death. [provided by RefSeq, May 2013]

Uniprot Description

ITGAV: The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. Belongs to the integrin alpha chain family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion

Cellular Component: cell surface; external side of plasma membrane; filopodium membrane; focal adhesion; integral to membrane; integrin complex; membrane; microvillus membrane; nucleus; plasma membrane

Molecular Function: extracellular matrix binding; fibronectin binding; insulin-like growth factor I binding; metal ion binding; opsonin binding; peptide binding; protease binding; protein heterodimerization activity; protein kinase C binding; receptor binding; voltage-gated calcium channel activity

Biological Process: angiogenesis; apoptotic cell clearance; blood vessel development; cell adhesion; cell differentiation; cell growth; cell migration; cell-matrix adhesion; cell-substrate adhesion; cellular calcium ion homeostasis; detection of molecule of bacterial origin; elevation of cytosolic calcium ion concentration; endothelial cell migration; entry of virus into host cell; fertilization; focal adhesion formation; gonad morphogenesis; heterotypic cell-cell adhesion; inflammatory response; integrin-mediated signaling pathway; multicellular organismal development; negative chemotaxis; negative regulation of lipid transport; negative regulation of lipoprotein metabolic process; negative regulation of low-density lipoprotein receptor biosynthetic process; positive regulation of cell adhesion; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of MAPKKK cascade; positive regulation of osteoblast proliferation; regulation of bone resorption; regulation of phagocytosis; release of sequestered calcium ion into cytosol; T cell activation