Integrin alpha V beta 6 Recombinant Protein | ITGAV&ITGB6 recombinant protein

Integrin alpha V beta 6 Protein, Mouse, Recombinant (DYKDDDDK & His Tag)

Cat. Num. AAA8123618

• Gene Names: Itgav; CD51; 1110004F14Rik; 2610028E01Rik; D430040G12Rik
• Purity: >90% as determined by SDS-PAGE
• Synonyms: Integrin alpha V beta 6; Integrin alpha V beta 6 Protein; Mouse; Recombinant (DYKDDDDK & His Tag); Mouse ITGAV & ITGB6 Heterodimer Protein (DYKDDDDK & His Tag); ITGAV & ITGB6 Protein; integrin; alpha 5 (fibronectin receptor; alpha polypeptide); ITGAV&ITGB6 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Val988&Met1-Pro708

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Phe 31 & Gly 22
• Tag: C-DYKDDDDK & C-His
• Protein Construction: A DNA sequence encoding the extracellular domain (Met 1-Val988) of mouse ITGAV (NP_032428.2) was fused with a DYKDDDDK tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met1-Pro708) of mouse ITGB6 (NP_067334.1) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the mouse ITGAV&ITGB6 heterodimer was purified.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for ITGAV&ITGB6 recombinant protein

Background: Integrin alpha-5, also known as ITGA5, is a single-pass type I membrane protein which belongs to the integrin alpha chain family. ITGA5 contains 7 FG-GAP repeats. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. ITGAV&ITGB6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of ITGAV&ITGB6 via clathrin-mediated endocytosis promotes carcinoma cell invasion.

References

Suzuki S., et al.,(1987), Amino acid sequence of the vitronectin receptor alpha subunit and comparative expression of adhesion receptor mRNAs. J. Biol. Chem. 262:14080-14085.Sims M.A., et al., (2000), Cloning and characterisation of ITGAV, the genomic sequence for human cell adhesion protein (vitronectin) receptor alpha subunit, CD51.Cytogenet. Cell Genet. 89:268-271.Ota T., et al.,(2004), Complete sequencing and characterization of 21,243 full-length human cDNAs.Nat. Genet. 36:40-45.

NCBI and Uniprot Product Information

• NCBI GI #: 154240716
• NCBI GeneID: 16410
• NCBI Accession #: NP_032428.2; NP_067334.1
• NCBI GenBank Nucleotide #: NM_008402.3
• UniProt Accession #: P43406; A2AKI6
• Molecular Weight: 115,360 Da
• NCBI Official Full Name: integrin alpha-V
• NCBI Official Synonym Full Names: integrin alpha V
• NCBI Official Symbol: Itgav
• NCBI Official Synonym Symbols: CD51; 1110004F14Rik; 2610028E01Rik; D430040G12Rik
• NCBI Protein Information: integrin alpha-V; vitronectin receptor alpha polypeptide (VNRA); vitronectin receptor subunit alpha
• UniProt Protein Name: Integrin alpha-V
• UniProt Gene Name: Itgav
• UniProt Entry Name: ITAV_MOUSE

NCBI Description

This gene encodes a protein that is a member of the integrin superfamily. Integrins are transmembrane receptors involved cell adhesion and signaling, and they are subdivided based on the heterodimer formation of alpha and beta chains. This protein has been shown to heterodimerize with beta 1, beta 3, beta 6 and beta 8. The heterodimer of alpha v and beta 3 forms the Vitronectin receptor. This protein interacts with several extracellular matrix proteins to mediate cell adhesion and may play a role in cell migration. In mouse, deficiency of this gene is associated with defects in vascular morphogenesis in the brain and early post-natal death. [provided by RefSeq, May 2013]

Uniprot Description

ITGAV: The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. Belongs to the integrin alpha chain family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis

Cellular Component: filopodium membrane; cell surface; focal adhesion; membrane; microvillus membrane; integral to membrane; plasma membrane; intracellular; cytosol; nucleus; integrin complex; external side of plasma membrane

Molecular Function: voltage-gated calcium channel activity; opsonin binding; insulin-like growth factor I binding; protease binding; protein kinase C binding; extracellular matrix binding; protein heterodimerization activity; metal ion binding; fibronectin binding; peptide binding; receptor binding

Biological Process: negative regulation of lipid transport; focal adhesion formation; entry of virus into host cell; positive regulation of cell adhesion; T cell activation; cell-matrix adhesion; multicellular organismal development; negative chemotaxis; gonad morphogenesis; regulation of phagocytosis; elevation of cytosolic calcium ion concentration; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; angiogenesis; cell growth; cell adhesion; inflammatory response; cell differentiation; integrin-mediated signaling pathway; blood vessel development; cell migration; regulation of bone resorption; negative regulation of lipoprotein metabolic process; detection of molecule of bacterial origin; cell-substrate adhesion; cellular calcium ion homeostasis; heterotypic cell-cell adhesion; fertilization; negative regulation of low-density lipoprotein receptor biosynthetic process; release of sequestered calcium ion into cytosol; positive regulation of osteoblast proliferation; endothelial cell migration; apoptotic cell clearance; positive regulation of cell migration