Human Integrin Alpha V (ITGaV) ELISA Kit | ITGaV elisa kit

Human Integrin Alpha V (ITGaV) ELISA Kit

Cat. Num. AAA2023065

• Gene Names: ITGAV; CD51; MSK8; VNRA; VTNR
• Reactivity: Human
• Synonyms: Integrin Alpha V (ITGaV); Human Integrin Alpha V (ITGaV) ELISA Kit; CD51; ITG-AV; MSK8; VNRA; Vitronectin Receptor; Alpha Polypeptide; ITGaV elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Integrin Alpha V (ITGaV).; No significant cross-reactivity or interference between Integrin Alpha V (ITGaV) and analogues was observed.
• Sequence Length: 1,002

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.059ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Integrin Alpha V (ITGaV) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Integrin Alpha V (ITGaV) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ITGaV elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Integrin Alpha V (ITGaV). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Integrin Alpha V (ITGaV). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Integrin Alpha V (ITGaV), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Integrin Alpha V (ITGaV) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for ITGaV elisa kit

CD & Adhesion molecule

NCBI and Uniprot Product Information

• NCBI GI #: 223468597
• NCBI GeneID: 3685
• NCBI Accession #: NP_001138472.1
• NCBI GenBank Nucleotide #: NM_001145000.2
• UniProt Accession #: P06756; Q53SK4; Q59EB7; Q6LD15; A0AV67; B0LPF4; B7Z883; B7ZLX0; D3DPG8; E7EWZ6
• Molecular Weight: 111,132 Da
• NCBI Official Full Name: integrin alpha-V isoform 3
• NCBI Official Synonym Full Names: integrin, alpha V
• NCBI Official Symbol: ITGAV
• NCBI Official Synonym Symbols: CD51; MSK8; VNRA; VTNR
• NCBI Protein Information: integrin alpha-V
• UniProt Protein Name: Integrin alpha-V
• Protein Family: Integrin
• UniProt Gene Name: ITGAV
• UniProt Synonym Gene Names: MSK8; VNRA
• UniProt Entry Name: ITAV_HUMAN

NCBI Description

This gene encodes a protein that is a member of the integrin superfamily. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This protein undergoes post-translational cleavage to yield disulfide-linked heavy and light chains that combine with multiple integrin beta chains to form different integrins. This protein has been shown to heterodimerize with beta 1, beta 3, beta 5, beta 6, and beta 8; the heterodimer of alpha v and beta 3 is the Vitronectin receptor. This protein interacts with several extracellular matrix proteins to mediate cell adhesion and may play a role in cell migration. It is proposed that this protein may regulate angiogenesis and cancer progression. Alternative splicing results in multiple transcript variants that encode different protein isoforms. Note that the integrin alpha 5 and integrin alpha V chains are produced by distinct genes. [provided by RefSeq, Jan 2015]

Uniprot Description

ITGAV: The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. Belongs to the integrin alpha chain family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q31-q32

Cellular Component: filopodium membrane; focal adhesion; cell surface; membrane; microvillus membrane; integral to plasma membrane; plasma membrane; phagocytic vesicle; integrin complex; external side of plasma membrane

Molecular Function: voltage-gated calcium channel activity; viral receptor activity; opsonin binding; protein binding; insulin-like growth factor I binding; protease binding; protein kinase C binding; extracellular matrix binding; transforming growth factor beta binding; fibronectin binding; metal ion binding

Biological Process: negative regulation of lipid transport; axon guidance; extracellular matrix organization and biogenesis; entry of virus into host cell; positive regulation of cell adhesion; cell-matrix adhesion; negative chemotaxis; entry of symbiont into host cell by promotion of host phagocytosis; regulation of phagocytosis; antigen processing and presentation of peptide antigen via MHC class I; positive regulation of cell proliferation; antigen processing and presentation of exogenous peptide antigen via MHC class I; angiogenesis; cell growth; cell adhesion; integrin-mediated signaling pathway; cell migration; negative regulation of lipoprotein metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; cell-substrate adhesion; heterotypic cell-cell adhesion; negative regulation of low-density lipoprotein receptor biosynthetic process; positive regulation of osteoblast proliferation; vascular endothelial growth factor receptor signaling pathway; blood coagulation; leukocyte migration; apoptotic cell clearance; positive regulation of cell migration