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Testing Data
Minimaly Concentration Assay Kit
Minimal Inhibitory Concentration Kit
Reactivity
Gram Negative Rods, E Coli, Other gram negative bacteria
Synonyms
Minimaly Concentration; Minimal Inhibitory Concentration Kit; MIC; MIC Kit; Gram Negative; MIC Assay; Gram Negative Rods; Minimaly Concentration assay kit
Reactivity
Gram Negative Rods, E Coli, Other gram negative bacteria
Samples
E Coli grown in LB.Pseudomonas aeruginosa, Staphylococcus aureushave not been evaluated, but are commonly tested.
Detection Method
Absorbance: OD 460nm
Features & Benefits
Broth Microdilution Assay
Quantification of any antimicrobial agent against a given bacterial isolate
Sensitive
Simple
Quick counting and Time-Course assay protocols
Determination of antibiotic susceptibility, MIC, resistance and viability in E Coli or other gram negative bacteria.
For Gram Negative Rods
Quantification of any antimicrobial agent against a given bacterial isolate
Sensitive
Simple
Quick counting and Time-Course assay protocols
Determination of antibiotic susceptibility, MIC, resistance and viability in E Coli or other gram negative bacteria.
For Gram Negative Rods
Preparation and Storage
Store at -20 degree C
Shelf life: ~12 months
Shelf life: ~12 months
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Related Product Information for Minimaly Concentration assay kit
Testing antibiotic susceptibility in bacterial isolates enables the detection of drug resistance in common bacterial pathogens. This ensures susceptibility to selected pharmaceutical interventions for specific pathogens classified as gram negative rods. The broth microdilution, a well-known method in microbiology is flexible and provides quantitative and semi-quantitative results to monitor resistance amongst bacterial pathogens. It is called microdilution because it involves the use of small volumes of broth dispensed in sterile, plastic microdilution trays (~0.1 ml of broth). Minimal Inhibitory Concentration (MIC) kit, testing for Gram Negative Rods (GNR) is a broth microdilution assay that is simple yet sensitive for the quantification of any antimicrobial agent against a given bacterial isolate. The antibiotic efficacy is quantified using a Water Soluble Tetrazolium salt (WST) which changes color reflecting the viability of the bacteria as a response to antibiotic treatment. The test is performed in a microplate with bacteria-inoculated-Luria Bertani (LB) broth and serial dilutions of antimicrobial agents can be added. Our kit includes four antibiotics often prescribed for infections caused by gram negative rods. They are: Ampicillin Sodium, Chloramphenicol, Enrofloxacin, and Gentamicin Sulfate. Vancomycin Hydrochloride, an antibiotic effective in the treatment of gram positive cocci, is included as a negative control. The Antibiotic Susceptibility Limits of these antibiotics have been evaluated and correlated with published standards (Andrews, 2001) for Minimum Inhibitory Concentrations.
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Product Notes
The Gram Negative Rods, E Coli, Other gram negative bacteria Minimaly Concentration (Catalog #AAA849574) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849574 Assay Kit recognizes Gram Negative Rods, E Coli, Other gram negative bacteria Minimaly Concentration. It is sometimes possible for the material contained within the vial of "Minimaly Concentration, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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