Interferon alpha 1/IFNA1 Recombinant Protein | IFNA1 recombinant protein

Interferon alpha 1/IFNA1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8118678

• Gene Names: IFNA1; IFL; IFN; IFNA@; IFNA13; IFN-ALPHA; IFN-alphaD
• Purity: >95 % as determined by SDS-PAGE
• Synonyms: Interferon alpha 1/IFNA1; Interferon alpha 1/IFNA1 Protein; Human; Recombinant (His Tag); Human IFN-alpha/IFNA1/IFN Protein (His Tag); interferon; alpha 1; IFL Protein; IFN Protein; IFN-ALPHA Protein; IFN-alphaD Protein; IFNA13 Protein; IFNA@ Protein; IFNA1 recombinant protein

• Host: Yeast
• Purity/Purification: >95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Sequence Positions: 176aa
• Sequence: Cys24-Glu189

• Species: Human
• Tag: C-His
• Predicted N Terminal: Cys24
• Protein Construction: |A DNA sequence encoding the human IFNA1 (NP_076918.1) (Cys24-Glu189) was expressed with a polyhistidine tag at the C-terminus
• Activity: Measured in antiviral assays using WISH cells infected with vesicular stomatitis virus. The ED50 for this effect is 40-200pg/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; Shipping: They are shipped out with blue ice pack

Testing Data

Testing Data

(Measured in antiviral assays using WISH cells infected with vesicular stomatitis virus. The ED50 for this effect is 40-200pg/mL.)

Related Product Information for IFNA1 recombinant protein

IFNA1, also known as IFN-alpha and IFNA, belongs to the alpha/beta interferon family. Interferons(IFNs) are proteins made and released by host cells in response to the presence of pathogens such as viruses, bacteria, parasites, or tumor cells. They belong to the large class of glycoproteins known as cytokines. IFNs stimulate the production of two enzymes: a protein kinase and an oligoadenylate synthetase. They allow for communication between cells to trigger the protective defenses of the immune system that eradicate pathogens or tumors. IFNs can activate immune cells, such as natural killer cells and macrophages; they increase recognition of infection or tumor cells by up-regulating antigen presentation to T lymphocytes, and they also increase the ability of uninfected host cells to resist new infection by the virus. Leukocyte interferon is produced predominantly by B lymphocytes. Immune interferon is produced by mitogen-or antigen-stimulated T lymphocytes. IFNA1 is produced by macrophages and has antiviral activities.

References

Takayama I, et al. (2012) The nucleocapsid protein of measles virus blocks host interferon response. Virology. 424(1):45-55.
Vairo D, et al. (2011) Severe impairment of IFN-? and IFN-? responses in cells of a patient with a novel STAT1 splicing mutation. Blood. 118(7):1806-17.
Bhattacharya S, et al. (2011) Bcr-abl signals to desensitize chronic myeloid leukemia cells to IFN? via accelerating the degradation of its receptor. Blood. 118(15):4179-87.

NCBI and Uniprot Product Information

• NCBI GI #: 13128950
• NCBI GeneID: 3439
• NCBI Accession #: NP_076918.1
• NCBI GenBank Nucleotide #: NM_024013.2
• Molecular Weight: 19.5 kDa
• NCBI Official Full Name: interferon alpha-1/13
• NCBI Official Synonym Full Names: interferon alpha 1
• NCBI Official Symbol: IFNA1
• NCBI Official Synonym Symbols: IFL; IFN; IFNA@; IFNA13; IFN-ALPHA; IFN-alphaD
• NCBI Protein Information: interferon alpha-1/13
• Protein Family: Interferon

NCBI Description

The protein encoded by this gene is produced by macrophages and has antiviral activity. This gene is intronless and the encoded protein is secreted. [provided by RefSeq, Sep 2011]