HDAC3/NCOR1 active protein

HDAC3/NCOR1 Complex (human), (recombinant)

Cat. Num. AAA566674

• Purity: >=90% (SDS-PAGE); Purified by multi-step chromatography
• Synonyms: HDAC3/NCOR1; HDAC3/NCOR1 Complex (human); (recombinant); HDAC3/NCOR1 active protein

• Host: Insect cells
• Purity/Purification: >=90% (SDS-PAGE); Purified by multi-step chromatography
• Form/Format: Liquid. In 50mM TRIS, pH8.0, 138 mM NaCl and 10% glycerol

• Source Note: Produced in a baculovirus expression system
• Specific Activity: >=150U/ug. One U=1 pmol/min at 37 degree C, 100uM, FLUOR DE LYS-SIRT1 deacetylase substrate
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: For long term storage, store at -80 degree C; Shipping: Shipped on Dry Ice

Related Product Information for HDAC3/NCOR1 active protein

HDAC3, although a class I HDAC, is found in co-repressor complexes with the SMRT/N-CoR proteins, complexes distinct from than those comprising other class I enzymes. It has a unique domain structure, including both nuclear localization and nuclear export sequences and has been assigned to its own phylogenetic subclass.  Inhibition of HDAC3 may be the determining factor in the anti-proliferative effects of HDAC inhibitors on cancer cells and its caspase-dependent cleavage and relocation to the cytoplasm may be critical to the progression of apoptosis. It has been shown that HDAC3, rather than the class II HDACs 4 and 5, likely diretly deacetylates MEF2, implying an important role in pathways affecting heart disease.

Product Categories/Family for HDAC3/NCOR1 active protein

Cellular Analysis; Cell Death; Stem Cells; Epigenetics; Histone Posttranslational Modifications; Proteostasis; Posttranslational Modifications; Proteolysis & Protein Degradation

NCBI and Uniprot Product Information

• UniProt Accession #: O15379; O75376
• Molecular Weight: 49/40kDa
• UniProt Protein Name: Histone deacetylase 3
• UniProt Gene Name: HDAC3
• UniProt Synonym Gene Names: HD3
• UniProt Entry Name: HDAC3_HUMAN

Uniprot Description

HDAC3: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation. Interacts with HDAC7 and HDAC9. Forms a heterologous complex at least with YY1. Interacts with DAXX, HDAC10 and DACH1. Found in a complex with NCOR1 and NCOR2. Component of the N-Cor repressor complex, at least composed of NCOR1, NCOR2, HDAC3, TBL1X, TBL1R, CORO2A and GPS2. Interacts with BCOR, MJD2A/JHDM3A, NRIP1, PRDM6 and SRY. Interacts with BTBD14B. Interacts with GLIS2. Interacts (via the DNA-binding domain) with NR2C1; the interaction recruits phosphorylated NR2C1 to PML bodies for sumoylation. Component of the Notch corepressor complex. Interacts with CBFA2T3 and NKAP. Interacts with APEX1; the interaction is not dependent on the acetylated status of APEX1. Interacts with and deacetylates MAPK14. Interacts with ZMYND15. Widely expressed. Belongs to the histone deacetylase family. HD type 1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Apoptosis; Nuclear receptor co-regulator; Transcription, coactivator/corepressor; Deacetylase; EC 3.5.1.98

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: nucleoplasm; Golgi apparatus; transcriptional repressor complex; spindle microtubule; cytoplasm; histone deacetylase complex; plasma membrane; nucleus

Molecular Function: protein binding; cyclin binding; NAD-dependent histone deacetylase activity (H3-K9 specific); enzyme binding; NAD-dependent histone deacetylase activity (H3-K14 specific); chromatin DNA binding; histone deacetylase binding; protein deacetylase activity; NAD-dependent histone deacetylase activity (H4-K16 specific); chromatin binding; histone deacetylase activity; transcription factor binding; transcription corepressor activity

Biological Process: circadian rhythm; negative regulation of JNK cascade; establishment and/or maintenance of chromatin architecture; Notch signaling pathway; transcription, DNA-dependent; nerve growth factor receptor signaling pathway; regulation of mitotic cell cycle; regulation of multicellular organism growth; regulation of protein stability; chromatin modification; spindle assembly; cellular lipid metabolic process; negative regulation of transcription from RNA polymerase II promoter; negative regulation of cell cycle; protein amino acid deacetylation; positive regulation of transcription factor import into nucleus; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; circadian regulation of gene expression; negative regulation of transcription, DNA-dependent; positive regulation of TOR signaling pathway; negative regulation of apoptosis