Typical Testing Data/Standard Curve (for reference only)
HDAC3 assay kit
HDAC3 Immunoprecipitation (IP) & Activity Assay Kit
Cell lysate and Nuclear Extract
Only kit on the market for both IP as well as measuring activity of HDAC3.
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Background: Histone deacetylases (HDACs) play a central role in controlling cell cycle regulation, cell differentiation, and tissue development. These proteins have crucial roles in development and physiology, especially in the heart and nervous system. They are also deeply involved in cellular proliferation, cell cycle and apoptosis. HDAC3 is primarily localized in the nucleus, but can also be found in the cytoplasm and at the plasma membrane. The HDAC3 IP & Activity Assay Kit provides an antibody-based method to specifically immunoprecipitate the HDAC3 complex from cells & tissues and to measure HDAC3 activity fluorometrically. HDA3 is immunoprecipitated from cell or nuclear extract(s) using HDAC3 antibody followed by capturing the complex on protein-A/G beads. The immunoprecipitated complex is incubated with the HDAC substrate. Only the deacetylated substrate is cleaved by the Developer to produce a fluorophore, which can be easily analyzed using a fluorescence plate reader.
Summary: Detection method - Fluorescence (Ex/Em=368/442 nm)
Applications: - Immunoprecipitation of HDAC3 complex from cell & nuclear extract(s) - Measurement of HDAC3 activity of immunoprecipitated complex/purified enzyme - Screening for activators or inhibitors of HDAC3
NCBI and Uniprot Product Information
NCBI Description
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene. [provided by RefSeq, Jul 2008]
Uniprot Description
HDAC3: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation. Interacts with HDAC7 and HDAC9. Forms a heterologous complex at least with YY1. Interacts with DAXX, HDAC10 and DACH1. Found in a complex with NCOR1 and NCOR2. Component of the N-Cor repressor complex, at least composed of NCOR1, NCOR2, HDAC3, TBL1X, TBL1R, CORO2A and GPS2. Interacts with BCOR, MJD2A/JHDM3A, NRIP1, PRDM6 and SRY. Interacts with BTBD14B. Interacts with GLIS2. Interacts (via the DNA-binding domain) with NR2C1; the interaction recruits phosphorylated NR2C1 to PML bodies for sumoylation. Component of the Notch corepressor complex. Interacts with CBFA2T3 and NKAP. Interacts with APEX1; the interaction is not dependent on the acetylated status of APEX1. Interacts with and deacetylates MAPK14. Interacts with ZMYND15. Widely expressed. Belongs to the histone deacetylase family. HD type 1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell cycle regulation; Nuclear receptor co-regulator; Deacetylase; Apoptosis; EC 3.5.1.98; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 5q31
Cellular Component: nucleoplasm; Golgi apparatus; transcriptional repressor complex; spindle microtubule; histone deacetylase complex; cytoplasm; plasma membrane; nucleus
Molecular Function: NAD-dependent histone deacetylase activity (H3-K9 specific); cyclin binding; protein binding; enzyme binding; NAD-dependent histone deacetylase activity (H3-K14 specific); chromatin DNA binding; histone deacetylase binding; protein deacetylase activity; NAD-dependent histone deacetylase activity (H4-K16 specific); chromatin binding; histone deacetylase activity; transcription corepressor activity; transcription factor binding
Biological Process: circadian rhythm; negative regulation of JNK cascade; establishment and/or maintenance of chromatin architecture; Notch signaling pathway; nerve growth factor receptor signaling pathway; transcription, DNA-dependent; regulation of mitotic cell cycle; regulation of multicellular organism growth; regulation of protein stability; cellular lipid metabolic process; chromatin modification; spindle assembly; negative regulation of transcription from RNA polymerase II promoter; negative regulation of cell cycle; protein amino acid deacetylation; positive regulation of transcription factor import into nucleus; positive regulation of transcription from RNA polymerase II promoter; circadian regulation of gene expression; positive regulation of protein amino acid phosphorylation; negative regulation of transcription, DNA-dependent; positive regulation of TOR signaling pathway; negative regulation of apoptosis
Research Articles on HDAC3
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Product Notes
The HDAC3 hdac3 (Catalog #AAA841874) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "HDAC3, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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