Annexin A3 (ANXA3) Recombinant Protein | ANXA3 recombinant protein

Recombinant Annexin A3 (ANXA3)

Cat. Num. AAA2009571

• Gene Names: ANXA3; ANX3
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Annexin A3 (ANXA3); Recombinant Annexin A3 (ANXA3); ANXA3 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-AIQKAIRGIG TDEKMLISIL TERSNAQRQL IVKEYQAAYG KELKDDLKGD LSGHFEHLMV ALVTPPAVFD AKQLKKSMKG AGTNEDALIE ILTTRTSRQM KDISQAYYTV YKKSLGDDIS SETSGDFRKA LLTLA
• Sequence Length: 323

• Applicable Applications for ANXA3 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Ala26~Ala160 (Accession # P12429) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 4826643
• NCBI GeneID: 306
• NCBI Accession #: NP_005130.1
• NCBI GenBank Nucleotide #: NM_005139.2
• UniProt Accession #: P12429; Q6LET2; B2R9W6
• Molecular Weight: 16.5kDa
• NCBI Official Full Name: annexin A3
• NCBI Official Synonym Full Names: annexin A3
• NCBI Official Symbol: ANXA3
• NCBI Official Synonym Symbols: ANX3
• NCBI Protein Information: annexin A3; PAP-III; annexin-3; lipocortin III; 35-alpha calcimedin; calcimedin 35-alpha; Annexin III (lipocortin III); placental anticoagulant protein III; inositol 1,2-cyclic phosphate 2-phosphohydrolase; annexin III (lipocortin III, 1,2-cyclic-inositol-phosphate phosphodiesterase, placental anticoagulant protein III, calcimedin 35-alpha)
• UniProt Protein Name: Annexin A3
• Protein Family: Annexin
• UniProt Gene Name: ANXA3
• UniProt Synonym Gene Names: ANX3; PAP-III
• UniProt Entry Name: ANXA3_HUMAN

NCBI Description

This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions in the inhibition of phopholipase A2 and cleavage of inositol 1,2-cyclic phosphate to form inositol 1-phosphate. This protein may also play a role in anti-coagulation. [provided by RefSeq, Jul 2008]

Uniprot Description

ANXA3: a calcium/phospholipid-binding protein and inhibitor of phospholipase A2, Possesses anti-coagulant properties. Cleaves the cyclic bond of inositol 1,2-cyclic phosphate to form inositol 1-phosphate. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 4q21.21

Cellular Component: specific granule; phagocytic vesicle membrane; membrane; cytoplasm; plasma membrane

Molecular Function: calcium-dependent phospholipid binding; phospholipase A2 inhibitor activity; calcium ion binding; calcium-dependent protein binding

Biological Process: positive regulation of angiogenesis; neutrophil degranulation; negative regulation of catalytic activity; defense response to bacterium; positive regulation of transcription factor activity; phagocytosis