beta-Secretase 1 - BACE-1 Active Protein | BACE1 active protein

Active Recombinant Human beta-Secretase 1 - BACE-1

Cat. Num. AAA845073

• Gene Names: BACE1; ASP2; BACE; HSPC104
• Applications: SDS-Page
• Purity: > or = 90% by SDS-PAGE
• Synonyms: beta-Secretase 1 - BACE-1; Active Recombinant Human beta-Secretase 1 - BACE-1; Beta-secretase 1; Aspartyl protease 2; ASP2; Beta-site amyloid precursor protein cleaving enzyme 1; Beta-site APP cleaving enzyme 1; Memapsin-2; Membrane-associated aspartic protease 2; Aspartic-like protease 56 kDa; Aspartyl protease 1; Beta-site amyloid precursor protein cleaving enzyme 2; Down region aspartic protease; Memapsin-1; Membrane-associated aspartic protease 1; Theta-secretase; BACE1 active protein

• Host: Insect cell
• Specificity: > or = 120 x 10³ units/mg
• Purity/Purification: > or = 90% by SDS-PAGE
• Form/Format: Supplied in PBS with 50% glycerol
• Sequence Length: 376

• Applicable Applications for BACE1 active protein: SDS-PAGE; HPLC
• Application Notes: Active recombinant human BACE-1 is useful in studying enzyme regulation, determining target substrates, screening BACE inhibitors, or as a positive control in BACE activity assays. MBS, Inc. recombinant BACE-1 produced in insect cells is about 200 times higher in specific activity relative to that produced in E. coli.
• Solubility/Reconstitution Instructions: Use 20 ul sterile D.I. water to dissolve 10 ug of protein for a final conc of 0.5 ug/ul. For further dilution, use 10 mM Tris, pH 8.0.
• Biological Activity: MyBioSource's recombinant human beta-Secretase/BACE-1 is biologically active as determined by using the beta-Secretase Activity Assay Kit.
• Unit Definition: One unit is defined as the amount of enzyme that produces 1 pmol/min EDANS by hydrolyzing the substrate BVT9964 (NH2-Arg-Glu(EDANS)-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys(DABCYL)-Arg-COOH) at 37 degree C in 100 mM acetate buffer pH 4.0.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: Stable for 1 year at –70°C. Avoid multiple freeze/thaw cycles as activity may decrease.

Testing Data

Related Product Information for BACE1 active protein

Beta-Secretase, also called BACE1 (beta-site of APP cleaving enzyme) or memapsin-2 is an aspartic-acid protease important in the pathogenesis of Alzheimer's disease, and in the formation of myelin sheaths in peripheral nerve cells. The transmembrane protein contains two active site aspartate residues in its extracellular protein domain.

Product Categories/Family for BACE1 active protein

Proteins and Enzymes; Proteins and Enzymes (A-Z) Proteins and Enzymes; Animal-Free Origin (AOF) Recombinant Proteins & Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 333755186
• NCBI GeneID: 23621
• NCBI Accession #: NP_001193978.1
• NCBI GenBank Nucleotide #: NM_001207049.1
• UniProt Accession #: P56817; Q9BYB9; Q9BYC0; Q9BYC1; Q9UJT5; Q9ULS1; A0M8W7; B0YIU9; E9PE65; H7BXJ9
• Molecular Weight: 47 kDa (mature protease domain, 46-460aa)
• NCBI Official Full Name: beta-secretase 1 isoform F
• NCBI Official Synonym Full Names: beta-site APP-cleaving enzyme 1
• NCBI Official Symbol: BACE1
• NCBI Official Synonym Symbols: ASP2; BACE; HSPC104
• NCBI Protein Information: beta-secretase 1
• UniProt Protein Name: Beta-secretase 1
• Protein Family: Beta-secretase
• UniProt Gene Name: BACE1
• UniProt Synonym Gene Names: BACE; KIAA1149; ASP2; Asp 2; Beta-site APP cleaving enzyme 1
• UniProt Entry Name: BACE1_HUMAN

NCBI Description

Cerebral deposition of amyloid beta peptide is an early and critical feature of Alzheimer's disease. Amyloid beta peptide is generated by proteolytic cleavage of amyloid precursor protein (APP) by two proteases, one of which is the protein encoded by this gene. The encoded protein, a member of the peptidase A1 protein family, is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. Multiple transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, May 2011]

Uniprot Description

BACE: an integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. Cleaves APP at the amino terminus of the A-beta peptide sequence, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated carboxy-terminal fragment which is later release by gamma-secretase. Four splice-variant isoforms have been described.

Protein type: Protease; Membrane protein, integral; EC 3.4.23.46

Chromosomal Location of Human Ortholog: 11q23.2-q23.3

Cellular Component: Golgi apparatus; multivesicular body; cytoplasmic vesicle membrane; cell surface; integral to plasma membrane; axon; endoplasmic reticulum; late endosome; integral to membrane; plasma membrane; trans-Golgi network; endosome

Molecular Function: protein binding; enzyme binding; beta-amyloid binding; beta-aspartyl-peptidase activity; aspartic-type endopeptidase activity

Biological Process: beta-amyloid metabolic process; membrane protein ectodomain proteolysis; proteolysis