BACE1 active protein

Human CellExp BACE1, human recombinant (Native)

Cat. Num. AAA844513

• Applications: SDS-Page
• Purity: >=98%
• Synonyms: BACE1; Human CellExp BACE1; human recombinant (Native); ASP2; BACE; FLJ90568; HSPC104; KIAA1149; Beta-secretase-1; memapsin-2; aspartyl-protease-2; beta-site-APP-cleaving-enzyme-1; BACE1 active protein

• Host: HEK293 cells
• Purity/Purification: >=98%
• Form/Format: Lyophilized from 0.22 mum filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.; Appearance: Lyophilized
• Sequence Length: 376

• Applicable Applications for BACE1 active protein: SDS-PAGE
• Solubility/Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.
• Endotoxin Level: <1 EU/mug by LAL method
• Biological Activity: Measured by its ability to cleave a fluorescent peptide substrate Mca-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Lys(Dnp)-Arg-Arg-NH2. Cleavage of the substrate can be measured using excitation and emission wavelengths of 320 and 405 nm, respectively. The specific activity is > 7.5 pmoles /min /mug.
• Results: Measured by its ability to cleave a fluorescent peptide substrate Mca-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Lys(Dnp)-Arg-Arg-NH2. Cleavage of the substrate can be measured using excitation and emission wavelengths of 320 and 405 nm, respectively. The specific activity is > 7.5 pmoles /min /mug.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for BACE1 active protein

Background: Beta-secretase 1 (BACE1), also known as beta-site APP cleaving enzyme 1 (beta-site amyloid precursor protein cleaving enzyme 1), memapsin-2 (membrane-associated aspartic protease 2), and aspartyl protease 2 (ASP2), beta-Secretase, and is a member of the peptidase A1 protein family, BACE1 is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. BACE1 is an aspartic-acid protease important in the pathogenesis of Alzheimer's disease, and in the formation of myelin sheaths in peripheral nerve cells.[1] The transmembrane protein contains two active site aspartate residues in its extracellular protein domain and may function as a dimer. This protease is responsible for the proteolytic processing of the amyloid precursor protein (APP). Generation of the 40 or 42 amino acid-long amyloid-beta peptides that aggregate in the brain of Alzheimer's patients requires two sequential cleavages of the APP. Extracellular cleavage of APP by BACE creates a soluble extracellular fragment and a cell membrane-bound fragment referred to as C99. The elevation of BACE1 levels can be induced by amyloid plaques surrounding neurons at early stages of pathology before neuron death occurs, and may drive a positive-feedback loop in AD.

Product Categories/Family for BACE1 active protein

Proteins and Enzymes; Human cell expressed recombinant proteinsCytokines; Growth Factors & Hormones; Human cell expressed recombinant proteinsCytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A-Z) Proteins and Enzymes; Cytokines; Growth Fa

NCBI and Uniprot Product Information

• NCBI GI #: 333755186
• NCBI GeneID: 23621
• NCBI Accession #: NP_001193978.1
• NCBI GenBank Nucleotide #: NM_001207049.1
• UniProt Accession #: P56817; Q9BYB9; Q9BYC0; Q9BYC1; Q9UJT5; Q9ULS1; A0M8W7; B0YIU9; E9PE65; H7BXJ9
• Molecular Weight: This protein contains no "tags" and has a calculated MW of 49 kDa. The predicted N-terminus is Thr22. DTT-reduced protein migrates as 50-65 kDa polypeptide in SDS-PAGE due to glycosylation.
• NCBI Official Full Name: beta-secretase 1 isoform F
• UniProt Protein Name: Beta-secretase 1
• Protein Family: Beta-secretase
• UniProt Gene Name: BACE1
• UniProt Synonym Gene Names: BACE; KIAA1149; ASP2; Asp 2; Beta-site APP cleaving enzyme 1
• UniProt Entry Name: BACE1_HUMAN

NCBI Description

Cerebral deposition of amyloid beta peptide is an early and critical feature of Alzheimer's disease. Amyloid beta peptide is generated by proteolytic cleavage of amyloid precursor protein (APP) by two proteases, one of which is the protein encoded by this gene. The encoded protein, a member of the peptidase A1 protein family, is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. Multiple transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, May 2011]

Uniprot Description

BACE: an integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. Cleaves APP at the amino terminus of the A-beta peptide sequence, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated carboxy-terminal fragment which is later release by gamma-secretase. Four splice-variant isoforms have been described.

Protein type: Protease; EC 3.4.23.46; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q23.2-q23.3

Cellular Component: multivesicular body; Golgi apparatus; cell surface; cytoplasmic vesicle membrane; endoplasmic reticulum; integral to plasma membrane; axon; late endosome; integral to membrane; plasma membrane; trans-Golgi network; endosome

Molecular Function: protein binding; enzyme binding; beta-amyloid binding; beta-aspartyl-peptidase activity; aspartic-type endopeptidase activity

Biological Process: beta-amyloid metabolic process; membrane protein ectodomain proteolysis; proteolysis