Rabbit anti-Human, Mouse Beta-secretase 1 Polyclonal Antibody | anti-BACE1 antibody

Beta-secretase 1 (CT)

Cat. Num. AAA395443

• Gene Names: BACE1; ASP2; BACE; HSPC104
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Antigen Immunoaffiinity Purification
• Synonyms: Beta-secretase 1; Polyclonal Antibody; Beta-secretase 1 (CT); BACE; KIAA1149; Beta-site amyloid precursor protein cleaving enzyme 1; anti-BACE1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Immunoaffiinity Purification
• Form/Format: Provided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative
• Sequence Length: 376

• Applicable Applications for anti-BACE1 antibody: Western Blot (WB)
• Immunogen: Synthetic peptide corresponding to amino acids 485 to 501 of human BACE. Sequence differs from that of rat and mouse by one amino acid.
• Positive Control: Human brain tissue lysate
• Preparation and Storage: Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles

Western Blot/Blotting

(Western blot analysis)

Related Product Information for anti-BACE1 antibody

Accumulation of the amyloid- (A) plaque in the cerebral cortex is a critical event in the pathogenesis of Alzheimer?s disease. A peptide is generated by proteolytic cleavage of the amyloid protein precursor (APP) at - and -sites by two proteases. APP is first cleaved by secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for secretase to generate the 4 kDa amyloid- peptide, which is deposited in the brains of all suffers of Alzheimer?s disease. The long-sought secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2)1-4. BACE/Asp2 is a novel transmembrane aspartic protease and colocalizes with APP.

Product Categories/Family for anti-BACE1 antibody

Polyclonal Antibody; Neuronal Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 333755186
• NCBI GeneID: 23621
• NCBI Accession #: NP_001193978.1
• NCBI GenBank Nucleotide #: NM_001207049.1
• UniProt Accession #: P56817; Q9BYB9; Q9BYC0; Q9BYC1; Q9UJT5; Q9ULS1; A0M8W7; B0YIU9; E9PE65; H7BXJ9
• Molecular Weight: 70
• NCBI Official Full Name: beta-secretase 1 isoform F
• NCBI Official Synonym Full Names: beta-site APP-cleaving enzyme 1
• NCBI Official Symbol: BACE1
• NCBI Official Synonym Symbols: ASP2; BACE; HSPC104
• NCBI Protein Information: beta-secretase 1; asp 2; memapsin-2; APP beta-secretase; aspartyl protease 2; beta-site APP cleaving enzyme 1; beta-secretase 1 precursor variant 1; transmembrane aspartic proteinase Asp2; membrane-associated aspartic protease 2; beta-site amyloid beta A4 precursor protein-cleaving enzyme
• UniProt Protein Name: Beta-secretase 1
• Protein Family: Beta-secretase
• UniProt Gene Name: BACE1
• UniProt Synonym Gene Names: BACE; KIAA1149; ASP2; Asp 2
• UniProt Entry Name: BACE1_HUMAN

NCBI Description

Cerebral deposition of amyloid beta peptide is an early and critical feature of Alzheimer's disease. Amyloid beta peptide is generated by proteolytic cleavage of amyloid precursor protein (APP) by two proteases, one of which is the protein encoded by this gene. The encoded protein, a member of the peptidase A1 protein family, is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. Multiple transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, May 2011]

Uniprot Description

Function: Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. Ref.15 Ref.24

Catalytic activity: Broad endopeptidase specificity. Cleaves Glu-Val-Asn-Leu-|-Asp-Ala-Glu-Phe in the Swedish variant of Alzheimer amyloid precursor protein.

Enzyme regulation: Inhibited by RTN3 and RTN4. Ref.20 Ref.21

Subunit structure: Monomer. Interacts with GGA1, GGA2 and GGA3. Interacts with RTN3 and RTN4. Interacts with SNX6. Interacts with PCSK9. Ref.19 Ref.20 Ref.21 Ref.22 Ref.23 Ref.24

Subcellular location: Membrane; Single-pass type I membrane protein. Golgi apparatus › trans-Golgi network. Endoplasmic reticulum. Endosome. Cell surface. Cytoplasmic vesicle membrane. Note: Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface. Ref.17 Ref.24

Tissue specificity: Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata. Ref.15 Ref.16

Domain: The transmembrane domain is necessary for its activity. It determines its late Golgi localization and access to its substrate, APP. Ref.17

Post-translational modification: Glycosylated. Ref.16

Sequence similarities: Belongs to the peptidase A1 family.

Sequence caution: The sequence BAA86463.2 differs from that shown. Reason: Frameshift at position 34.