Interleukin-4 Recombinant Protein | IL-4 recombinant protein

Human Interleukin-4 Recombinant

Cat. Num. AAA553112

• Purity: >95%, as determined by SDS-PAGE and HPLC
• Synonyms: Interleukin-4; Human Interleukin-4 Recombinant; il4; il-4; interleukin-4; B-cell stimulatory factor 1; bsf-1; IL-4 recombinant protein

• Host: E Coli
• Purity/Purification: >95%, as determined by SDS-PAGE and HPLC
• Form/Format: Interleukin-4 was lyophilized from a 0.2 um filtered solution in 2.5% glycine, 0.5% sucrose, 0.01% Tween80, 5 mM Glutamic acid, pH 4.5.
• Sequence: MGLTSQLLPP LFFLLACAGN FVHGHKCDIT LQEIIKTLNS LTEQKTLCTE LTVTDIFAAS KNTTEKETFC RAATVLRQFY SHHEKDTRCL GATAQQFHRH KQLIRFLKRL DRNLWGLAGL NSCPVKEANQ STLENFLERL KTIMREKYSK CSS
• Sequence Length: 153

• Domain: four helix cytokine core
• Host Note: Optimized DNA sequence encoding Human Interleukin-4 mature chain was expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Molecular Weight Note: Native human Interleukin-4, generated by the proteolytic removal of the signal peptide and propeptide,the molecule has a calculated molecular mass of approximately 15 kDa.; ; Recombinant Interleukin-4 is a disulfide-linked monomer protein consisting of 130 amino acid residue subunits, migrates as an approximately 15 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for IL-4 recombinant protein

Interleukin-4 is produced mainly by a subpopulation of activated T-cells (Th2) which are the biologically most active helper cells for B-cells and which also secrete IL-5 and IL6. The biological activities of IL-4 are mediated by a specific receptor The extracellular domain of the IL4 receptor is related to the receptors for EPO, IL6, and the beta chain of the IL2 receptor. It has been given the name CD124. Two types of IL4 receptor (IL4R) exist: the type 1 receptor is a heterodimer consisting of CD132 and IL4R-alpha. The type 2 receptor is a heterodimer consisting of IL4R-alpha and IL13R-alpha- 1. IL4 enhances expression of MHC class 2 antigens on B- cells. It can promote their capacity to respond to other B-cell stimuli and to present antigens for T- cells.Pretreatment of macrophages with IL4 prevents the production of IL1, TNF-alpha and prostaglandins in response to activation of the cells by bacterial endotoxins or IFN-gamma.

Product Categories/Family for IL-4 recombinant protein

Cytokines

References

• pathogenicity of a disease-associated human IL-4 receptor allele in experimental asthma .J. Exp. Med., Sep 2009; 206: 2191 - 2204.
• complex regulation of tartrate-resistant acid phosphatase (trap) expression by interleukin 4 (IL-4) : IL-4 indirectly suppresses receptor activator of nf -kappab ligand (rankl) - mediated trap expression but modestly induces its expression directly .J. Biol. Chem., Sep 2009; 10.1074/jbc.M109.001016.
• IL-4r, a new member that associates with syk kinase: implication in IL-4-induced human neutrophil functions .J. Immunol., Sep 2009; 10.4049/jimmunol.0900109.
• gm-csf and IL-4 induce dendritic cell differentiation and disrupt osteoclastogenesis through m-csf receptor shedding by up-regulation of tnf- converting enzyme (tace) .Blood, Sep 2009; 10.1182/blood-2009-04-215020.
• ship represses the generation of il-3-induced m2 macrophages by inhibiting IL-4 production from basophils .J. Immunol., Sep 2009; 183: 3652 - 3660.