Immunohistochemistry
Rabbit anti-Human IL-4 Polyclonal Antibody | anti-IL-4 antibody
Rabbit Anti Human IL-4 Polyclonal
Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml.
IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml.
Immunohistochemical analysis of IL-4 expression in formalin fixed paraffin embedded sections of Human esophageal squamous cell carcinoma.
It was used as primary antibody, dilution 1:200.
Upon reconstitution, this antibody can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
Shelf Life: >12 months
Immunohistochemistry
Immunohistochemistry
• complex regulation of tartrate-resistant acid phosphatase (trap) expression by interleukin 4 (IL-4) : IL-4 indirectly suppresses receptor activator of nf -kappab ligand (rankl) - mediated trap expression but modestly induces its expression directly .J. Biol. Chem., Sep 2009; 10.1074/jbc.M109.001016.
• gm-csf and IL-4 induce dendritic cell differentiation and disrupt osteoclastogenesis through m-csf receptor shedding by up-regulation of tnf- converting enzyme (tace) .Blood, Sep 2009; 10.1182/blood-2009-04-215020.
• ship represses the generation of il-3-induced m2 macrophages by inhibiting IL-4 production from basophils .J. Immunol., Sep 2009; 183: 3652 - 3660.
• mycobacterium tuberculosis culture filtrate proteins plus cpg-odn confer protection to bcg primed mice by inhibiting IL-4 secretion .Infect. Immun., Sep 2009; 10.1128/IAI.00580-09.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
Uniprot Description
IL4: Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Genetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Belongs to the IL-4/IL-13 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis; Cell cycle regulation; Cytokine
Chromosomal Location of Human Ortholog: 5q31.1
Cellular Component: extracellular space; external side of plasma membrane
Molecular Function: protein binding; growth factor activity; cytokine activity; interleukin-4 receptor binding
Biological Process: regulation of isotype switching; negative regulation of osteoclast differentiation; positive regulation of isotype switching to IgG isotypes; positive regulation of transcription, DNA-dependent; microglial cell activation; regulation of proton transport; female pregnancy; chemotaxis; positive regulation of activated T cell proliferation; response to organic cyclic substance; positive regulation of interleukin-10 production; positive regulation of isotype switching to IgE isotypes; B cell costimulation; connective tissue growth factor biosynthetic process; positive regulation of MHC class II biosynthetic process; positive regulation of B cell proliferation; positive regulation of T cell proliferation; positive regulation of interleukin-13 production; negative regulation of macrophage activation; response to nutrient; response to drug; cholesterol metabolic process; negative regulation of chronic inflammatory response; regulation of immune response; T-helper 2 type immune response; B cell activation; regulation of phosphorylation; negative regulation of nitric oxide biosynthetic process; negative regulation of acute inflammatory response; defense response to protozoan; T-helper 1 cell lineage commitment; response to ethanol; positive regulation of chemokine biosynthetic process; innate immune response in mucosa; positive regulation of tyrosine phosphorylation of Stat5 protein; positive regulation of T cell differentiation; retina development in camera-type eye; B cell differentiation; response to cytokine stimulus; cellular defense response; immune response; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; T-helper 2 cell differentiation; negative regulation of transcription, DNA-dependent; positive regulation of defense response to virus by host; negative regulation of apoptosis
Disease: Stroke, Ischemic
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Product Notes
The IL-4 il4 (Catalog #AAA551020) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Anti Human IL-4 Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IL-4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), IHC (Immunohistochemistry), ELISA (EIA). ELISA: Use at a concentration range of 1-2ug/ml. Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml. IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml. Immunohistochemical analysis of IL-4 expression in formalin fixed paraffin embedded sections of Human esophageal squamous cell carcinoma. It was used as primary antibody, dilution 1:200. Researchers should empirically determine the suitability of the IL-4 il4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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