Typical Testing Data/Standard Curve (for reference only)
Human Interleukin-36 alpha ELISA Kit | IL36A elisa kit
Human Interleukin-36 alpha ELISA Kit
Gene Names
IL36A; FIL1; FIL1E; IL1F6; IL-1F6; IL1(EPSILON); FIL1(EPSILON)
Reactivity
Human
Synonyms
Interleukin-36 alpha; Human Interleukin-36 alpha ELISA Kit; FIL1 epsilon; Interleukin-1 epsilon; IL-1 epsilon; Interleukin-1 family member 6; IL-1F6; IL36A; FIL1E; IL1E; IL1F6; IL36A elisa kit
Reactivity
Human
Specificity
This assay recognizes recombinant and natural human Interleukin-36 alpha. No significant cross-reactivity or interference was observed.
Sequence Length
158
Assay Type
Sandwich
Detection Range
0.156-10 ng/mL
Sensitivity
0.078ng/mL
Intra-Assay CV
<=5.3%
Inter-Assay CV
<=7.8%
Recovery
99%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for IL36A elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of human Interleukin-36 alpha concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin-36 alpha. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Interleukin-36 alpha and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Interleukin-36 alpha, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Interleukin-36 alpha in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin-36 alpha. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Interleukin-36 alpha and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Interleukin-36 alpha, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Interleukin-36 alpha in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,684 Da
NCBI Official Full Name
interleukin-36 alpha
NCBI Official Synonym Full Names
interleukin 36, alpha
NCBI Official Symbol
IL36A
NCBI Official Synonym Symbols
FIL1; FIL1E; IL1F6; IL-1F6; IL1(EPSILON); FIL1(EPSILON)
NCBI Protein Information
interleukin-36 alpha
UniProt Protein Name
Interleukin-36 alpha
Protein Family
UniProt Gene Name
IL36A
UniProt Synonym Gene Names
FIL1E; IL1E; IL1F6; IL-1 epsilon; IL-1F6
UniProt Entry Name
IL36A_HUMAN
NCBI Description
The protein encoded by this gene is a cytokine that can activate NF-kappa-B and MAPK signaling pathways to generate an inflammatory response. The encoded protein functions primarily in skin and demonstrates increased expression in psoriasis. In addition, decreased expression of this gene has been linked to a poor prognosis in both hepatocellular carcinoma and colorectal cancer patients. [provided by RefSeq, Nov 2015]
Uniprot Description
IL1F6: Belongs to the IL-1 family.
Protein type: Cytokine
Chromosomal Location of Human Ortholog: 2q12-q14.1
Cellular Component: extracellular region; extracellular space
Molecular Function: cytokine activity; interleukin-1 receptor binding
Biological Process: cytokine and chemokine mediated signaling pathway; immune response; inflammatory response to antigenic stimulus; innate immune response; positive regulation of interleukin-6 production
Research Articles on IL36A
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Product Notes
The Human IL36A il36a (Catalog #AAA2881247) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2881247 ELISA Kit recognizes Human IL36A. It is sometimes possible for the material contained within the vial of "Interleukin-36 alpha, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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