Human Interleukin-36 alpha ELISA Kit | IL36A elisa kit

Human Interleukin-36 alpha ELISA Kit

Cat. Num. AAA760191

• Gene Names: IL36A; FIL1; FIL1E; IL1F6; IL-1F6; IL1(EPSILON); FIL1(EPSILON)
• Reactivity: Human
• Synonyms: Interleukin-36 alpha; Human Interleukin-36 alpha ELISA Kit; IL36A (Interleukin-36 alpha)/FIL1 epsilon/IL-1E/IL1F6/FIL1/FIL1 epsilon/FIL1 (EPSILON)/FIL1E/IL-1 epsilon/IL1 (EPSILON)/IL1E/IL1F6/IL-1F6/IL-1F6 (FIL-1-epsilon)/interleukin 1 family; member 6 (epsilon)/interleukin 1; epsilon/Interleukin 36; Alpha/Interleukin-1 epsilon/interleukin-1 family member 6/Interleukin-36 Alpha; IL36A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of IL36A. No significant cross-reactivity or interference between IL36A and analogues was observed.
• Sequence Length: 158

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL36A elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 7657092
• NCBI GeneID: 27179
• NCBI Accession #: NP_055255.1
• NCBI GenBank Nucleotide #: NM_014440.2
• Molecular Weight: 17,684 Da
• NCBI Official Full Name: interleukin-36 alpha
• NCBI Official Synonym Full Names: interleukin 36, alpha
• NCBI Official Symbol: IL36A
• NCBI Official Synonym Symbols: FIL1; FIL1E; IL1F6; IL-1F6; IL1(EPSILON); FIL1(EPSILON)
• NCBI Protein Information: interleukin-36 alpha
• UniProt Protein Name: Interleukin-36 alpha
• Protein Family: Interleukin
• UniProt Gene Name: IL36A
• UniProt Synonym Gene Names: FIL1E; IL1E; IL1F6; IL-1 epsilon; IL-1F6
• UniProt Entry Name: IL36A_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that can activate NF-kappa-B and MAPK signaling pathways to generate an inflammatory response. The encoded protein functions primarily in skin and demonstrates increased expression in psoriasis. In addition, decreased expression of this gene has been linked to a poor prognosis in both hepatocellular carcinoma and colorectal cancer patients. [provided by RefSeq, Nov 2015]

Uniprot Description

IL1F6: Belongs to the IL-1 family.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: 2q12-q14.1

Cellular Component: extracellular region; extracellular space

Molecular Function: cytokine activity; interleukin-1 receptor binding

Biological Process: cytokine and chemokine mediated signaling pathway; immune response; inflammatory response to antigenic stimulus; innate immune response; positive regulation of interleukin-6 production