Typical Testing Data/Standard Curve (for reference only)
Mouse Interleukin 6 ELISA Kit | IL-6 elisa kit
Mouse Interleukin 6 ELISA Kit
Gene Names
Il6; ILg6; Ifnb2
Reactivity
Mouse
Synonyms
Interleukin 6; Mouse Interleukin 6 ELISA Kit; IL-6 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of IL-6. No significant cross-reactivity or interference between IL-6 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IL-6 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive or Sandwich
Sensitivity
1.0pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for IL-6 elisa kit
Intended Uses: This IL-6 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse IL-6. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: IL-6 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for IL-6. Standards or samples are then added to the microtiter plate wells and IL-6 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IL-6 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for IL-6 are added to each well to "sandwich" the IL-6 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-6 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-6 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: IL-6 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for IL-6. Standards or samples are then added to the microtiter plate wells and IL-6 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IL-6 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for IL-6 are added to each well to "sandwich" the IL-6 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-6 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-6 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for IL-6 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24,357 Da
NCBI Official Full Name
interleukin-6
NCBI Official Synonym Full Names
interleukin 6
NCBI Official Symbol
Il6
NCBI Official Synonym Symbols
ILg6; Ifnb2
NCBI Protein Information
interleukin-6; IL-6; Interleukin 6 (interferon, beta 2)
UniProt Protein Name
Interleukin-6
UniProt Gene Name
Il6
UniProt Synonym Gene Names
Il-6; IL-6
UniProt Entry Name
IL6_RAT
NCBI Description
a cytokine involved in development and possibly in neurodegenerative processes [RGD, Feb 2006]
Uniprot Description
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation ().
Research Articles on IL-6
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Product Notes
The Mouse IL-6 il6 (Catalog #AAA730957) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA730957 ELISA Kit recognizes Mouse IL-6. It is sometimes possible for the material contained within the vial of "Interleukin 6, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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